Monoclonal antibodies with specificity for fetal erythroid cells

• US 7,858,757 B2
• Filed: 03/31/2005
• Issued: 12/28/2010
• Est. Priority Date: 03/31/2004
• Status: Active Grant

• Current Assignee: KellBenx Inc.
• Original Assignee: AdnaGen AG
• Inventors: Hollmann, Christiane, Albert, Winfried, Zimmermann, Silke, Stachelhaus, Stefan
• Primary Examiner(s): GABEL, GAILENE
• Application Number:
  US10/599,512
• Publication Number:
  US 20070275418A1
• Time in Patent Office: 2,098 Days
• Field of Search: 530/380, 530/386, 530/388.1, 530/388.2, 530/388.22, 530/388.23, 435/7.2, 435/7.25, 435/287.2, 436/523, 436/526, 436/547, 436/548, 436/63, 436/175, 436/177, 436/811
• US Class Current: 530/388.22
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C07K 2317/14 Specific host cells or culture conditions, e.g. components, pH or temperature
• CPC Class Codes: G01N 2333/70582 CD71
• CPC Class Codes: G01N 2469/10 Detection of antigens from microorganism in sample from host
• CPC Class Codes: G01N 33/5094 for blood cell populations red blood cells G01N33/80
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: G01N 33/577 involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens; G01N33/53 - G01N33/576 take precedence
• CPC Class Codes: G01N 33/80 involving blood groups or blood types or red blood cells white blood cells G01N33/56972
• CPC Class Codes: Y10T 436/25125 Digestion or removing interfering materials
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

The present invention concerns a monoclonal antibody and corresponding hybridoma cells and antigens suitable for isolating fetal cells from maternal blood. The monoclonal antibody reacts with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cell.

Claims

1. A monoclonal antibody reacting with a surface antigen present on fetal red blood cells and their nucleated precursor cells, but not with CD71 or other surface antigens on adult erythroid cells.

2. The antibody according to claim 1, having reactivity with fetal erythroid cells and their nucleated precursor cells, which express the CD71 antigen but are negative for CD45 antigen expression.

4. A method for detection or identification of fetal red blood cells in a sample comprising labeling said fetal red blood cells and their nucleated precursor cells with a monoclonal antibody according to claim 1.

5. The method according to claim 4, wherein the sample is maternal blood.

6. The method according to claim 4, where said fetal red blood cells and their nucleated precursor cells are, subsequent to the detection and identification, further analyzed for a chromosomal or genetic defects.

7. The method according to claim 4, further comprising separating fetal red blood cells and their nucleated precursor cells binding the monoclonal antibody by flow cytometry, solid phase separation, immunomagnetic bead separation, or panning on plastic surfaces.

3. A hybridoma cell producing a monoclonal antibody reacting with a surface antigen present on fetal red blood cells and their nucleated precursor cells, but not with CD71 or other surface antigens on adult erythroid cells.