Trophic factor combinations for nervous system treatment

• US 7,862,826 B2
• Filed: 05/19/2008
• Issued: 01/04/2011
• Est. Priority Date: 08/27/2004
• Status: Expired due to Fees

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Golder, Francis J., Mitchell, Gordon S., McAnulty, Jonathan F., Murphy, Christopher J.
• Primary Examiner(s): Kolker; Daniel E
• Assistant Examiner(s): MACFARLANE, STACEY NEE
• Application Number:
  US12/123,366
• Publication Number:
  US 20090298741A1
• Time in Patent Office: 960 Days
• Field of Search: None
• US Class Current: 424/198.1
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/046 Tachykinins, e.g. eledoisins, substance P; Related peptides
• CPC Class Codes: A61K 38/1709 from mammals
• CPC Class Codes: A61K 38/185 Nerve growth factor [NGF]; Brain derived neurotrophic factor [BDNF]; Ciliary neurotrophic factor [CNTF]; Glial derived neurotrophic factor [GDNF]; Neurotrophins, e.g. NT-3
• CPC Class Codes: A61K 38/30 Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2 insulin-like growth factor binding protein A61K38/1754
• CPC Class Codes: A61K 9/06 Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels composition of ointments, creams or gels A61K47/00
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/404 Biocides, antimicrobial agents, antiseptic agents
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence

Abstract

The present invention relates to a composition including an effective amount of at least one of an antimicrobial peptide and a substance having an antimicrobial peptide effect and an effective amount of a neurotrophin. The composition can also include an effective amount of at least one of a growth factor and a neuropeptide. The present invention also relates a method of treating an injury to a nervous system of an animal that includes the steps of identifying the injury to the nervous system and applying to the injury an effective amount of at least one of antimicrobial peptide and a substance having an antimicrobial peptide effect. The method can also include applying an effective amount of one or more trophic factors selected from the group consisting of a growth factor, a neurotrophin, and a neuropeptide to the injury.

Claims

1. A method of treating an injury to a nervous system of an animal, the method comprising:

applying to the injury an effective amount of an antimicrobial peptide, BDNF, IGF-1, and Substance P, wherein the antimicrobial peptide is BNP-1 and wherein the injury comprises an acute spinal cord injury.

2. The method of claim 1, wherein a viscous substance is applied with the BNP-1, BDNF, IGF-1, and Substance P.

3. The method of claim 2, wherein the viscous substance comprises a polysaccharide.

4. The method of claim 2, wherein the viscous substance comprises hyaluronic acid.

5. The method of claim 1, wherein the BNP-1, BDNF, IGF-1, and Substance P are delivered in a slow release formula.

6. The method of claim 5, wherein the slow release formula is a matrix.

7. The method of claim 6, wherein the matrix includes a viscous substance.

8. The method of claim 6, wherein the matrix is a hydrogel.

9. The method of claim 8, wherein the hydrogel is a polymer matrix modified to contain a bifunctional poly(alkylene glycol) molecule covalently bonded to the polymer matrix.

10. The method of claim 8, wherein the hydrogel is cross-linked.

11. The method of claim 10, wherein the hydrogel is cross-linked with glutaraldehyde.

12. The method of claim 10, wherein the hydrogel is cross-linked via an interpenetrating network of one or more photopolymerizable acrylates.

13. The method of claim 8, wherein the BNP-1, BDNF, IGF-1, and Substance P are incorporated into the hydrogel.

14. The method of claim 13, wherein the BNP-1, BDNF, IGF-1, and Substance P are incorporated into the hydrogel through covalent bonds to poly(alkylene glycol) molecules of the hydrogel.

15. The method of claim 13, wherein the BNP-1, BDNF, IGF-1, and Substance P are incorporated into the hydrogel through entertainment within the hydrogel.

16. The method of claim 6, wherein the matrix is a collagen gel matrix.

17. The method of claim 16, wherein the collagen gel matrix is impregnated with the BNP-1, BDNF, IGF-1, and Substance P.

18. The method of claim 1, wherein the BNP-1, BDNF, IGF-1, and Substance P are applied with a medium with spaced supports.

19. The method of claim 18, wherein the spaced supports are selected from the group consisting of sponges, gels, and biopolymers.