Analysis of circulating tumor cells, fragments, and debris
First Claim
1. A method for monitoring malignancy in a test subject comprising:
- a. obtaining a blood sample from a test subject, said sample comprising a mixed cell population suspected of containing intact malignant cancer cells of epithelial cell origin and further comprising;
i. cell fragments derived from said malignant cells, and/orii. cellular debris derived from said malignant cells;
b. preparing a sample with magnetically-labeled said intact malignant cells, said cell fragments and said cellular debris wherein said blood sample is mixed with colloidal magnetic particles, having a size range between 90 to 150 nm and a bovine serum albumin coating using high temperature, coupled to a first biospecific ligand which reacts and binds specifically to an epitope present in each of said intact malignant cells, said cell fragments and said cellular debris to form specific binding complexes with said colloidal magnetic particles and first biospecific ligand;
c. exposing said specific binding complexes formed in step b) to an externally-applied high gradient magnetic field to the substantial exclusion of other specimen components;
d. contacting said specific binding complexes in step c) with at least one additional biospecific ligand forming a specific binding pair with a receptor of said intact malignant cells, said cell fragments and said cellular debris, to the substantial exclusion of other specimen components, wherein the receptor is present in malignant tumor cells of epithelial cell origin;
e. differentially analyzing amounts of said labeled malignant cells, said labeled cell fragments and said labeled cellular debris in step d) over time, a change in the numerical proportions of said labeled malignant cells, said labeled cell fragments, and said labeled cellular debris indicating a change of malignancy.
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Abstract
The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CellSpotter® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.
144 Citations
16 Claims
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1. A method for monitoring malignancy in a test subject comprising:
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a. obtaining a blood sample from a test subject, said sample comprising a mixed cell population suspected of containing intact malignant cancer cells of epithelial cell origin and further comprising; i. cell fragments derived from said malignant cells, and/or ii. cellular debris derived from said malignant cells; b. preparing a sample with magnetically-labeled said intact malignant cells, said cell fragments and said cellular debris wherein said blood sample is mixed with colloidal magnetic particles, having a size range between 90 to 150 nm and a bovine serum albumin coating using high temperature, coupled to a first biospecific ligand which reacts and binds specifically to an epitope present in each of said intact malignant cells, said cell fragments and said cellular debris to form specific binding complexes with said colloidal magnetic particles and first biospecific ligand; c. exposing said specific binding complexes formed in step b) to an externally-applied high gradient magnetic field to the substantial exclusion of other specimen components; d. contacting said specific binding complexes in step c) with at least one additional biospecific ligand forming a specific binding pair with a receptor of said intact malignant cells, said cell fragments and said cellular debris, to the substantial exclusion of other specimen components, wherein the receptor is present in malignant tumor cells of epithelial cell origin; e. differentially analyzing amounts of said labeled malignant cells, said labeled cell fragments and said labeled cellular debris in step d) over time, a change in the numerical proportions of said labeled malignant cells, said labeled cell fragments, and said labeled cellular debris indicating a change of malignancy. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for monitoring malignancy in a test subject comprising:
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a. obtaining a blood sample from a test subject, said sample comprising a mixed cell population suspected of containing intact malignant cancer cells of epithelial cell origin and clusters of said malignant cells; b. preparing a sample with magnetically-labeled said intact malignant cells and said clusters of malignant cells wherein said blood sample is mixed with colloidal magnetic particles, having a size range between 90 to 150 nm and a bovine serum albumin coating using high temperature, coupled to a first biospecific ligand which reacts and binds specifically to an epitope present in each of said intact malignant cells and said clusters of malignant cells to form specific binding complexes with said colloidal magnetic particles and first biospecific ligand; c. exposing said specific binding complexes formed in step b) to an externally-applied high gradient magnetic field to the substantial exclusion of other specimen components; d. contacting said specific binding complexes in step c) with at least one additional biospecific ligand forming a specific binding pair with a receptor of said intact malignant cells and said clusters of malignant cells, to the substantial exclusion of other specimen components, wherein the receptor is present in malignant tumor cells of epithelial cell origin; e. differentially analyzing amounts of said labeled malignant cells and said labeled clusters of malignant cells in step d) over time, a change in the numerical proportions of said labeled malignant cells and said labeled clusters of malignant cells indicating a change of malignancy. - View Dependent Claims (10, 11, 12, 13)
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14. A kit for assaying a blood sample suspected of containing intact malignant cancer cells of epithelial cell origin for the presence of malignant cells, cell fragments derived from malignant cells and cellular debris derived from malignant cells, comprising:
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a. coated colloidal magnetic nanoparticles comprising; i. a magnetic core material having a size range between 90 to 150 nm; ii. a protein base coating comprising bovine serum albumin applied using high temperature; and iii. an antibody that binds specifically to an epitope present in each of said intact malignant cells, said cell fragments and said cellular debris, wherein said antibody is coupled to said protein base coating on the colloidal magnetic nanoparticle; b. at least one antibody having binding specificity for a receptor of said malignant cell, said cell fragments and said cellular debris, and wherein the receptor is present in malignant tumor cells of epithelial cell origin; and c. an agent capable of staining further morphological features of said malignant cells, said cell fragments and said cellular debris. - View Dependent Claims (15, 16)
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Specification