Detecting element and detection method

• US 7,863,051 B2
• Filed: 01/18/2005
• Issued: 01/04/2011
• Est. Priority Date: 01/23/2004
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Yamamichi, Junta
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): Handy; Dwayne K
• Application Number:
  US10/554,219
• Publication Number:
  US 20060272433A1
• Time in Patent Office: 2,177 Days
• Field of Search: 422/68.1, 422/82.01, 422100-102, 436/63, 436/86, 436/149, 436/150, 436/174, 436/177, 436/179, 436/180, 436/164, 436/172
• US Class Current: 436/164
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/25625 Dilution
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A detection method for detecting a plurality of different substances contained in a specimen using a label, includes sequentially the steps of flowing the specimen through a detecting element having a first substance trapping portion immobilizing a first substance trapping body, a second substance trapping portion immobilizing a second substance trapping body, and a channel, and flowing a solution containing the label through the first substance trapping portion immobilizing the first substance trapping body and the second substance trapping portion immobilizing the second substance trapping body. Additional steps include flowing a solution for generating a signal from the label through the first substance trapping portion immobilizing the label to thereby acquire a signal from, and flowing a solution for generating a signal from the label through the second substance trapping portion immobilizing the label to thereby acquire a signal.

Claims

1. A detection method for detecting a plurality of different substances contained in a specimen using a same label, comprising sequentially the steps of:

flowing the specimen having a first substance and a second substance through a detecting element having a first substance trapping portion immobilizing a first substance trapping body for specifically trapping and immobilizing the first substance contained in the specimen, a second substance trapping portion immobilizing a second substance trapping body for specifically trapping and immobilizing the second substance contained in the specimen, the second substance being different from the first substance, and a channel, with the first substance trapping portion being different than the second substance trapping portion;

flowing a solution containing the label through the first substance trapping portion immobilizing the first substance trapping body and the second substance trapping portion immobilizing the second substance trapping body, the label comprising a first group of label molecules bonded with a third substance trapping body capable of specifically acting on the first substance and a second group of label molecules bonded with a fourth substance trapping body capable of specifically acting on the second substance;

flowing a solution for generating a signal from the label through the first substance trapping portion immobilizing the label such that a first layer of aqueous solution flow through the first substance trapping portion and a second layer of aqueous solution flow through the second substance trapping portion coexist while a third layer of alcoholic solution flow exists between the first layer of aqueous solution flow and the second layer of aqueous solution flow and that the solution for generating a signal from the label forms the first layer of aqueous solution flow, to thereby acquire a signal from the first substance trapping portion; and

flowing a solution for generating a signal from the label through the second substance trapping portion immobilizing the label such that a first layer of aqueous solution flow through the first substance trapping portion and a second layer of aqueous solution flow through the second substance trapping portion coexist while a third layer of alcoholic solution flow exists between the first layer of aqueous solution flow and the second layer of aqueous solution flow and that the solution for generating a signal from the label forms the second layer of aqueous solution flow, to thereby acquire a signal from the second substance trapping portion.

2. A detection method according to claim 1, wherein the label is an enzyme and the solution for generating a signal from the label is a solution containing a substrate for the enzyme.

3. A detection method according to claim 1, wherein the label is pH-sensitive fluorescent dye and the solution for generating a signal from the label is a solution having a pH which changes a fluorescent characteristic of the pH-sensitive fluorescent dye.