Method and assay for detection of residues
First Claim
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1. A method for detecting an analyte in a test solution comprising:
- a) configuring a binder for generating a detectable signal, the binder capable of combining with an analyte from the solution to form a binder-analyte complex;
b) immobilizing, within a test zone of a test strip, a test zone capture agent;
c) immobilizing, within a single control zone of a strip, a plurality of control zone capture agents, each of the plurality of control zone capture agents having different affinity to the binder and each able to capture the binder;
d) applying the binder to the test strip;
e) applying the test solution to the test strip, wherein the test solution and the binder combines to allow binding of analyte to binder to form a binder-analyte complex;
f) capturing binder unbound by analyte in the test zone;
g) capturing the binder, whether or not the binder has formed a binder-analyte complex, at the control zone;
h) comparing the signal in the control zone to the signal in the test zone to determine the presence of analyte in the solution,wherein a greater signal in the control zone, as compared to the test zone, indicates a positive result.
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Abstract
Embodiments described herein include methods and assays for detecting an analyte in a sample using a plurality of control zone capture agents. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone. Test results are determined by comparison of the control zone signal to a test zone signal.
67 Citations
28 Claims
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1. A method for detecting an analyte in a test solution comprising:
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a) configuring a binder for generating a detectable signal, the binder capable of combining with an analyte from the solution to form a binder-analyte complex; b) immobilizing, within a test zone of a test strip, a test zone capture agent; c) immobilizing, within a single control zone of a strip, a plurality of control zone capture agents, each of the plurality of control zone capture agents having different affinity to the binder and each able to capture the binder; d) applying the binder to the test strip; e) applying the test solution to the test strip, wherein the test solution and the binder combines to allow binding of analyte to binder to form a binder-analyte complex; f) capturing binder unbound by analyte in the test zone; g) capturing the binder, whether or not the binder has formed a binder-analyte complex, at the control zone; h) comparing the signal in the control zone to the signal in the test zone to determine the presence of analyte in the solution, wherein a greater signal in the control zone, as compared to the test zone, indicates a positive result. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method for detecting a plurality of analytes present in a test solution comprising:
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a) configuring a plurality of binders for generating a detectable signal, the binders capable of combining with an analyte from the solution to form a binder-analyte complex; b) immobilizing, within a first test zone of a test strip, a first test zone capture agent; c) immobilizing, within a second test zone of a test strip, a second test zone capture agent; d) immobilizing, within a single control zone of a strip, a plurality of control zone capture agents, each of the plurality of control zone capture agents having different affinity and each able to attach to at least one of the binders, whether or not the binder has formed a binder-analyte complex; e) applying the plurality of binders to the test strip; f) applying the test solution to the test strip, wherein the test solution and the plurality of binders combine to allow binding of analyte to binder to form a binder-analyte complex; g) capturing binder unbound by analyte in either or both the first test zone and second test zone; h) capturing the binder, whether or not the binder has formed a binder-analyte complex, at the control zone; i) comparing the signal in the control zone to the signals in the test zones to determine the presence of analyte in the solution, wherein a greater signal in the control zone, as compared to any one test zone, indicates a positive result. - View Dependent Claims (27, 28)
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Specification