Breeding plants

• US 7,866,091 B2
• Filed: 08/04/2008
• Issued: 01/11/2011
• Est. Priority Date: 02/05/2003
• Status: Expired due to Fees

• Current Assignee: Cropdesign N.V. (BASF SE)
• Original Assignee: Cropdesign N.V. (BASF SE)
• Inventors: Dillen, Willy, Lejeune, Pierre, Leyns, Frederik, Broekaert, Willem
• Primary Examiner(s): Palo; Francis T
• Application Number:
  US12/185,471
• Publication Number:
  US 20090035782A1
• Time in Patent Office: 890 Days
• Field of Search: 47/581.SE
• US Class Current: 47/58.10R
• CPC Class Codes: A01G 31/042 with containers travelling on a belt or the like, or conveyed by chains
• CPC Class Codes: A01G 9/143 Equipment for handling produce in greenhouses
• CPC Class Codes: Y02A 40/25 Greenhouse technology, e.g. cooling systems therefor
• CPC Class Codes: Y02P 60/21 Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

A process for breeding plants which comprises growing plants of a species in an array of containers charged with growing medium of uniform characteristics in an environment of controlled climatic conditions with controlled supply of nutrients and feed water and changing the positions of the containers within the environment as required to ensure at least substantially uniform exposure of all plants in the containers to conditions in the environment. A process for the breeding of open pollinating plants in a greenhouse environment is also provided. A process for breeding plants which comprises identifying trait leads.

Claims

1. A method for the efficient analysis of the effect of a gene of interest on a selected phenotypic parameter in a plant, the method comprising:

a) providing a population of plants grown in an environmentally controlled greenhouse environment;

b) providing a device for evaluation of the phenotypic parameter;

c) using the evaluation device to obtain information on the phenotypic parameter for each plant and then select and evaluate the information for the phenotypic parameter in each plant;

d) comparing the phenotypic parameter of the plants; and

e) statistically analyzing the differences for the phenotypic parameter between plants to identify trait leads.

2. The method of claim 1, wherein the plant is a transgenic plant.

3. The method of claim 1, wherein the plant is corn or rice.

4. The method of claim 3, wherein the corn is a fast cycling corn line.

5. The method of claim 4, wherein the corn is Gaspe.

6. The method of claim 1, wherein each plant within the population of plants is associated with information about the plant'"'"'s identity and greenhouse location.

7. The method of claim 1, wherein the selected phenotypic parameter is a yield component trait or a stress tolerance trait.

8. The method of claim 7, wherein the yield component trait is growth or yield.

9. The method of claim 1 in which the plants are transported on a conveyor system through a station with the evaluation equipment.

10. A method for the efficient analysis of a trait in transgenic plants, the method comprising:

a) providing recipient plant cells from a plant line for transformation;

b) obtaining a plurality of nucleotide vectors;

c) introducing the plurality of nucleotide vectors into the recipient plant cells to create a plurality of transgenic plants;

d) allowing the plurality of transgenic plants to grow in an environmentally controlled greenhouse environment,

e) analyzing each transgenic plant for the trait by visual evaluation or by use of appropriate equipment; and

f) comparing the trait for the transgenic plants to determine the effect of the nucleotide vectors on the trait.

11. The method of claim 10, wherein the trait is a yield, growth, or a stress tolerance trait.

12. The method of claim 10, wherein the plant line that is the source of recipient plant cells is corn or rice.

13. The method of claim 12, wherein the corn is a fast cycling corn line.

14. The method of claim 13, wherein the corn is Gaspe.

15. The method of claim 10 in which the plants are transported on a conveyor system through a station with the evaluation equipment.

16. A method for the efficient analysis of the effect of a gene of interest on a selected phenotypic parameter in plants, the method comprising the steps of:

a) providing a population of transgenic plants possessing the gene of interest and grown in an environmentally controlled greenhouse environment;

b) using an analyzing device to take and evaluate information on each plant and then analyze the information to obtain phenotypic data for each plant; and

c) performing a statistical analysis of the phenotypic data to analyze the effect of the gene of interest on the selected phenotypic parameter.

17. The method of claim 16, wherein the plant is corn or rice.

18. The method of claim 17, wherein the corn is a fast cycling corn line.

19. The method of claim 18, wherein the corn is Gaspe.

20. The method of claim 16 including as a first step introducing a transgene construct possessing at least one gene of interest into recipient plant cells to produce a population of transgenic plants.

21. The method of claim 20, wherein the plants cells are from corn or rice.

22. The method of claim 16, wherein the statistical analysis comprises the step of:

a) comparing the phenotypic parameter for the population of transgenic plants;

b) statistically analyzing the differences between transgenic plants for the phenotypic parameter; and

c) identifying trait leads.

23. The method of claim 16, wherein each plant within the population of plants is associated with information about the plant'"'"'s identity and greenhouse location.

24. The method of claim 16, wherein the selected phenotypic parameter is a yield component trait or a stress tolerance trait.

25. The method of claim 24, wherein the yield component trait is growth or yield.

26. The method of claim 16 in which the plants are transported on a conveyor system through a station with the evaluation equipment.

27. The method of claim 16, further comprising providing a plurality of plants and comparing the phenotypic parameter for a single member of the population of transgenic plants with the phenotypic parameter of the plurality of plants obtained by the analyzing device.

28. A method for the rapid analysis of the effect of a gene of interest on a selected phenotypic parameter in plants, the method comprising:

a) providing a population of transgenic plants possessing the gene of interest and grown in an environmentally controlled greenhouse environment;

b) pollinating the transgenic plant with the same variety of plant;

c) assaying the plants to identify plants possessing the gene of interest;

d) using an analyzing device to take and evaluate information on each plant and then analyze the information to obtain phenotypic data for each plant; and

e) performing a statistical analysis of the phenotypic data to analyze the effect of the gene of interest on the selected phenotypic parameter.

29. The method of claim 28, wherein the plant is corn.

30. The method of claim 29, wherein the corn is a fast cycling corn line.

31. The method of claim 30, wherein the corn is Gaspe.