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Method and apparatus for automated pre-treatment and processing of biological samples

  • US 7,867,443 B2
  • Filed: 07/07/2005
  • Issued: 01/11/2011
  • Est. Priority Date: 07/23/2004
  • Status: Active Grant
First Claim
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1. A method for automated antigen retrieval and staining of a biological sample on a slide, comprising the steps of:

  • providing an apparatus comprising;

    a heatable processing tank configured to perform antigen retrieval upon the sample,a slide holder configured to hold the slide anda moveable probe configured to administer reagents to the sample during staining, wherein the moveable probe is further configured to deliver reagents to additional samples on additional slides;

    mounting the slide onto the slide holder;

    rotating the slide about an axis parallel to a plane of the slide into a first orientation;

    causing the sample to contact a solution heated within the processing tank;

    removing the slide from the processing tank after a duration of time appropriate for antigen retrieval;

    rotating the slide about an axis parallel to a plane of the slide into a second orientation; and

    administering the reagents to the sample with the probe, wherein the solution comprises at least one chelating agent, at least one detergent and at least 50% by volume of at least one liquid that facilitates antigen retrieval.

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