Methods for in vivo evaluation of pulmonary physiology and/or function using NMR signals of polarized 129Xe
First Claim
1. An in vivo method for evaluating whether a subject has a respiratory disorder, or a cardiopulmonary disorder, comprising:
- delivering polarized 129Xe in vivo to a subject such that the polarized 129Xe travels across the alveolar-capillary membrane to be taken up in the blood across the membrane, the polarized gas in the blood having a polarized 129Xe NMR chemical shift signal frequency;
destroying the polarization of the polarized 129Xe in the blood and the membrane;
obtaining an NMR spectroscopic signal of the polarized gas in the subject over time at the blood and/or tissue resonant frequency to generate at least one dynamic data set to generate at least one NMR 129Xe uptake curve at least one chemical shift frequency of interest of signal strength values over time; and
evaluating the NMR derived dynamic data set to assess whether the subject has a respiratory or cardiopulmonary disorder.
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Abstract
In certain embodiments, methods of the present invention obtain dynamic data sets of an NMR spectroscopy signal of polarized 129Xe in a selected structure, environment, or system. The signal data can be used to evaluate: (a) the physiology of a membrane or tissue; (b) the operational condition or function of a body system or portion thereof (when at rest or under stimulation); and/or (c) the efficacy of a therapeutic treatment used to treat a diagnosed disorder, disease, or condition. Thus, the present invention provides methods for screening and/or diagnosing a respiratory, cardiopulmonary disorder or disease such as chronic heart failure, and/or methods for monitoring the efficacy of therapeutics administered to subject to treat the disorder or disease.
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Citations
27 Claims
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1. An in vivo method for evaluating whether a subject has a respiratory disorder, or a cardiopulmonary disorder, comprising:
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delivering polarized 129Xe in vivo to a subject such that the polarized 129Xe travels across the alveolar-capillary membrane to be taken up in the blood across the membrane, the polarized gas in the blood having a polarized 129Xe NMR chemical shift signal frequency; destroying the polarization of the polarized 129Xe in the blood and the membrane; obtaining an NMR spectroscopic signal of the polarized gas in the subject over time at the blood and/or tissue resonant frequency to generate at least one dynamic data set to generate at least one NMR 129Xe uptake curve at least one chemical shift frequency of interest of signal strength values over time; and evaluating the NMR derived dynamic data set to assess whether the subject has a respiratory or cardiopulmonary disorder. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. An in vivo method for evaluating a subject for chronic heart failure comprising:
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(a) delivering polarized 129Xe in vivo to a subject such that the polarized 129Xe moves across the alveolar-capillary membrane to be taken up in the blood across the membrane, the polarized gas in the blood having a corresponding polarized gas NMR chemical shift signal frequency; (b) destroying the polarization of the polarized 129Xe in the blood and the membrane; (c) obtaining an NMR spectroscopic signal of the polarized 129Xe in the subject over time at the blood chemical shift frequency to generate at least one dynamic data set of the NMR spectroscopic signal strength over time; (d) evaluating the dynamic data; and (e) determining whether the subject has chronic heart failure based on the obtaining and evaluating steps.
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Specification