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Priming module for microfluidic chips

  • US 7,867,776 B2
  • Filed: 05/06/2004
  • Issued: 01/11/2011
  • Est. Priority Date: 03/02/2001
  • Status: Active Grant
First Claim
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1. A method for simultaneously preparing a plurality of separation networks in a microfluidic device for a separation, each separation network being externally and fluidly accessible through a priming reservoir and a sample reservoir, comprising the steps of:

  • (a) dispensing a separation medium into one or more of the priming reservoirs fluidly connected to the plurality of the separation networks;

    (b) sealing a priming block against the one or more priming reservoirs;

    (c) driving separation medium from the one or more priming reservoirs into the plurality of separation networks with the priming block to simultaneously fill the separation networks with the separation medium;

    (d) transferring a plurality of samples from a sample array to the sample reservoirs in fluid connection with the plurality of filled separation networks, thereby preparing the plurality of separation networks contained in the microfluidic device for a separation; and

    (e) after performing steps (a) through (d), transferring the microfluidic device to an analyzer for separation and analysis of the prepared separation networks.

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