Nanoscale moiety placement methods

• US 7,867,782 B2
• Filed: 10/19/2006
• Issued: 01/11/2011
• Est. Priority Date: 10/19/2006
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Barth, Phillip W.
• Primary Examiner(s): YANG, NELSON C
• Application Number:
  US11/583,285
• Publication Number:
  US 20080096287A1
• Time in Patent Office: 1,545 Days
• Field of Search: None
• US Class Current: 436/518
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393

Abstract

Methods and structures for placing nanoscale moieties on substrates are provided.

Claims

1. A method of placing a nanomoiety at a deterministic location on a substrate, the method comprising:

preparing a nanomoiety having a first characteristic dimension;

providing a substrate having a surface;

providing a nanoscale binding site at a deterministic location on the surface, the binding site having a binding patch, the binding site having a second characteristic dimension larger than the first characteristic dimension of the nanomoiety;

associating the nanomoiety with a nanoscale delivery vehicle, the delivery vehicle having a third characteristic dimension larger than the first characteristic dimension of the nanomoiety, one of the nanoscale moiety and the nanoscale delivery vehicle having a binding region capable of binding at the binding patch;

exposing the surface to a fluid delivery medium containing the nanomoiety associated with the delivery vehicle;

stochastically contacting the binding region to the binding patch thereby binding the delivery vehicle to the substrate; and

rendering the nanoscale moiety from the delivery vehicle to leave the nanoscale moiety attached to the substrate, the nanoscale moiety having a retention characteristic for continued attachment to the substrate after rendering.

2. The method of claim 1, wherein the second characteristic dimension of the binding site is larger than the third characteristic dimension of the delivery vehicle.

3. The method of claim 1, wherein the second characteristic dimension of the binding site is smaller than the third characteristic dimension of the delivery vehicle.

4. The method of claim 1, wherein a plurality of nanomoieties are present in the delivery medium, each nanomoiety being associated with a delivery vehicle.

5. The method of claim 1, wherein a plurality of nanoscale binding sites are present on the surface, wherein each binding site has a binding patch, wherein each binding site has the second characteristic dimension larger than the first characteristic dimension of the nanomoiety.

6. The method of claim 5, wherein each nanoscale binding site is prepared at a deterministic location.

7. The method of claim 1, further including preparing a location layer on the surface of the substrate, the location layer having a hole therein, wherein during the step of exposing of the surface to a fluid delivery medium containing the nanomoiety associated with the delivery vehicle, the hole in conjunction with the delivery vehicle provides a location function for the delivery vehicle at the binding site.

8. The method of claim 1, further including disposing a location and alignment layer on the surface of the substrate, the location and alignment layer having a hole therein, wherein during the step of exposing of the surface to a fluid delivery medium containing the nanomoiety associated with the delivery vehicle, the hole in conjunction with the delivery vehicle provides a location and alignment function for the delivery vehicle at the binding site.

9. The method of claim 1, further including providing a reporter entity associated with one of the delivery vehicle and the nanomoiety.

10. The method of claim 9, further including detecting the reporter entity after one of the step of stochastically binding and the step of rendering.

11. The method of claim 1, wherein a multiplicity of nanoscale sites is prepared on the surface of the substrate, wherein each site has the third characteristic dimension greater than the first characteristic dimension of the nanomoiety, each site having a binding patch exposed to a delivery medium containing a multiplicity of nanomoieties, wherein each nanomoiety is associated with one of a multiplicity of delivery vehicles.

12. The method of claim 11, wherein a plurality of reporter entities is present in the delivery medium, each reporter entity being associated with one of a nanomoiety and a delivery vehicle.

13. The method of claim 11, wherein the number of nanomoieties present in the delivery medium is greater than the number of nanoscale sites on the surface of the substrate.

14. The method of claim 11, further including washing the surface of the substrate to remove any unbound delivery vehicles after one of the step of stochastically binding and the step of rendering.

15. The method of claim 7, further including removing the location layer after one of the step of stochastically binding and the step of rendering.

16. The method of claim 8, further including removing the location and alignment layer after one of the step of stochastically binding and the step of rendering.

17. The method of claim 4, wherein the shape of each delivery vehicles precludes more than one binding region at a time from binding at the binding patch.

18. The method of claim 11, wherein the shape of each delivery vehicle precludes more than one binding region at a time from binding at each of the multiplicity of binding patches.

19. The method of claim 11, wherein the third characteristic dimension of each delivery vehicle is less than the second characteristic dimension of each binding site and greater than half of the second characteristic dimension of each binding site.

20. The method of claim 11, wherein the third characteristic dimension of each delivery vehicle is greater than twice the second characteristic dimension of each binding site.

21. The method of claim 1, wherein the binding patch includes a first nucleotide and the binding region includes a second nucleotide, wherein the first nucleotide and the second nucleotide are complementary.

22. The method of claim 21, further comprising a second nanomoiety that is associated with a second nanoscale delivery vehicle, wherein one of the second nanomoiety and the second nanoscale delivery vehicle has a second binding region, wherein the substrate has a second nanoscale binding site at a deterministic location on the surface of the substrate, the second binding site having a second binding patch, wherein the binding patch includes a third nucleotide and the binding region includes a fourth nucleotide, wherein the third nucleotide and the fourth nucleotide are complementary, and wherein each of the first nucleotide and the second nucleotide are not complementary for either of the third nucleotide and the fourth nucleotide.

23. The method of claim 1, wherein the nanoscale moiety is a seed particle.

24. The method of claim 23, further comprising:

forming a mandrel on the seed particle;

forming a plurality of layers on the substrate around the mandrel, wherein the layers alternate between an insulator layer and a conductor layer; and

forming a nanohole with a diameter of the mandrel by removing the mandrel and the seed particle.

25. The method of claim 1, wherein the nanoscale moiety is a mandrel.

26. The method of claim 25, further comprising:

forming a plurality of layers on the substrate around the mandrel, wherein the layers alternate between an insulator layer and a conductor layer; and

forming a nanohole with a diameter of the mandrel by removing the mandrel.