Apparatus and method for performing ligand binding assays on microarrays in multiwell plates
First Claim
1. Apparatus for performing binding ligand assays, comprising a planar transparent plate including at least one well therein, said at least one well having at least a first planar wall oriented out of the plane of said plate, the first planar wall having a plurality of ligands formed thereon in an array,wherein said planar transparent plate includes a plurality of wells organized in rows and columns, and further wherein said rows are spaced apart a distance t given by the formula
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Abstract
An apparatus and method for real time, label-free imaging and quantitation of binding events at an array of positions are provided. Total internal reflection from a planar side wall of a well of a multiwell plate is used to create an evanescent field in the plane of a pattern of ligands immobilized on the wall. Embodiments include imaging and multiple analyte detection and quantitation of a single wall of a single well as well as the simultaneous imaging and multiple analyte detection and quantitation of a number of wells.
86 Citations
17 Claims
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1. Apparatus for performing binding ligand assays, comprising a planar transparent plate including at least one well therein, said at least one well having at least a first planar wall oriented out of the plane of said plate, the first planar wall having a plurality of ligands formed thereon in an array,
wherein said planar transparent plate includes a plurality of wells organized in rows and columns, and further wherein said rows are spaced apart a distance t given by the formula
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6. An apparatus for sensing and/or measuring quantitatively binding events between analytes and ligands, comprising:
a first transparent planar plate having an index of refraction n2 and at least one well having a height h and a wall thickness t that satisfies the following equation; - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
Specification