Method for eliminating crosstalk between waveguide grating-based biosensors located in a microplate and the resulting microplate
First Claim
1. A method for eliminating crosstalk of coupled light between a plurality of waveguide grating-based biosensors which are located in bottoms of a plurality of wells in a microplate, said method comprising the step of:
- isolating a waveguide within each biosensor from other waveguides that are within other biosensors to eliminate the crosstalk of coupled light between each of the biosensors, wherein the waveguide within each biosensor has coupled light propagating therein, and wherein the plurality of biosensors are formed within and are part of the bottoms of the plurality of wells within the microplate.
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Abstract
The present invention includes several methods for modifying the current processes of manufacturing optical sensing microplates that use continuous waveguide films to reduce/eliminate crosstalk between the biosensors that are incorporated within wells. The methods include (1) physically deteriorating/removing the waveguide film between individual biosensors; (2) chemically depositing highly absorbing materials within the waveguide film between individual biosensors; (3) patterning disordered (scattering) regions between the diffraction gratings that define individual biosensors; (4) using a specific mask and depositing individual patches of waveguide film, where each patch defines at least one biosensor. Each of these methods and several other methods described herein prevent the propagation of light between individual sensing regions, thereby eliminating optical crosstalk between the biosensors. The present invention also includes the resulting microplate.
44 Citations
31 Claims
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1. A method for eliminating crosstalk of coupled light between a plurality of waveguide grating-based biosensors which are located in bottoms of a plurality of wells in a microplate, said method comprising the step of:
isolating a waveguide within each biosensor from other waveguides that are within other biosensors to eliminate the crosstalk of coupled light between each of the biosensors, wherein the waveguide within each biosensor has coupled light propagating therein, and wherein the plurality of biosensors are formed within and are part of the bottoms of the plurality of wells within the microplate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 26, 27)
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10. A microplate comprising:
a frame including a plurality of wells formed therein, wherein each well has a bottom that incorporates a waveguide grating-based biosensor, wherein the plurality of biosensors are formed within and are part of the bottoms of the plurality of wells, wherein each biosensor is isolated from the other biosensors by isolating a waveguide within each biosensor from other waveguides that are within other biosensors to eliminate crosstalk of coupled light between each of the biosensors, wherein the waveguide within each biosensor has coupled light propagating therein, and wherein the plurality of biosensors are formed within and are part of the bottoms of the plurality of wells within the microplate. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 28, 29)
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19. A slide comprising:
a flat base having a plurality of waveguide grating-based biosensors formed therein, wherein each biosensor is isolated from the other biosensors by isolating a waveguide within each biosensor from other waveguides that are within other biosensors to eliminate crosstalk of coupled light between each of the biosensors, wherein the waveguide within each biosensor has coupled light propagating therein. - View Dependent Claims (20, 21, 22, 23, 24, 25, 30, 31)
Specification