System, method, and product for scanning of biological materials

• US 7,871,812 B2
• Filed: 10/27/2004
• Issued: 01/18/2011
• Est. Priority Date: 03/15/2002
• Status: Active Grant

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Oleink-Ovod, Ksenia, Weiner, Nathan K., Miles, Christopher, Schultz, Eric, Bukys, Albert, Katz, Joel M., Bradbury, David, DiPaolo, Roger, Odoy, Patrick J., Stura, David A., Regan, Thomas P., Jones, Mark, McKenzie, Eric Earl, Deweerd, Herman, Overbeck, James, Woolaver, Tim
• Primary Examiner(s): Griffin; Walter D
• Assistant Examiner(s): EDWARDS, LYDIA E
• Application Number:
  US10/975,788
• Publication Number:
  US 20050057676A1
• Time in Patent Office: 2,274 Days
• Field of Search: 435/287.2
• US Class Current: 435/287.2
• CPC Class Codes: G01N 2021/6471 Special filters, filter wheel
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.

Claims

1. A probe array cartridge transport assembly apparatus comprising:

a cartridge magazine, having an open top and an open bottom, capable of holding one or more probe array cartridges, wherein each probe array cartridge comprises at least one barcode, a planar substrate and an array of biological molecules covalently bound to a surface of the substrate;

a cartridge transport assembly comprising a cartridge holder, wherein the cartridge holder comprises at least one motor and a pitch and roll mechanism, and wherein the cartridge transport assembly is capable of transporting a probe array cartridge from the cartridge magazine to a scanner and back to the cartridge magazine and is further capable of advancing the cartridge magazine by one or more positions to a second probe array cartridge;

a barcode reader; and

a scanner.

2. The apparatus according to claim 1, wherein:

the cartridge magazine is housed within a cooled storage chamber.

3. The apparatus according to claim 1, wherein:

the cartridge magazine is advanced, by a linear motor, by one or more positions to a second probe array cartridge.

4. The apparatus according to claim 2, wherein:

the cooled storage chamber comprises a temperature and humidity controlled environment.

5. The apparatus according to claim 4, wherein:

the temperature and humidity controlled environment comprises a temperature for maintaining biological integrity.

6. The apparatus according to claim 5, wherein:

the temperature for maintaining biological integrity comprises a range between about 2°

C. and about 15°

C.

7. The apparatus according to claim 1, wherein:

the probe array cartridges are arranged in a vertical orientation.

8. The apparatus according to claim 1, wherein:

the cartridge magazine holds at least 48 probe array cartridges.

9. The apparatus according to claim 1, wherein:

the cartridge magazine holds at least 100 probe array cartridges.

10. The apparatus according to claim 1, wherein:

the cartridge magazine is substantially circular.

11. The apparatus according to claim 1, further comprising:

a warm thermal chamber constructed and arranged to reduce condensation on at least one of the one probe array cartridges by warming.

12. The apparatus according to claim 1, wherein:

the cartridge transport assembly comprises one or more elements that reversibly engage and disengage at least one of the probe array cartridges.

13. The apparatus according to claim 12, wherein:

the one or more elements engage one or more fiducial features.

14. A method of storing and transporting probe array cartridges between a scanner and a cartridge magazine, the method comprising:

storing at least one probe array cartridge in the cartridge holder according to claim 1, wherein each probe array cartridge comprises a planar substrate and an array of biological molecules covalently bound to a surface of the substrate;

transporting a probe array cartridge from the cartridge magazine to a scanner and back to the cartridge magazine;

and advancing the cartridge magazine by one or more positions to a second probe array cartridge.

15. The method according to claim 14, wherein:

the act of storing comprises providing a temperature and humidity controlled environment.

16. The method according to claim 15, wherein:

the temperature and humidity controlled environment comprises maintaining the at least one probe array at a temperature for maintaining biological integrity.

17. The method according to claim 16, wherein:

the temperature for maintaining biological integrity comprises a range between about 2°

C. and about 15°

C.

18. The method according to claim 14, wherein:

the probe array cartridges are arranged in a vertical orientation.

19. The method according to claim 14, wherein:

the cartridge magazine holds at least 48 probe array cartridges.

20. The method according to claim 14, wherein:

the cartridge magazine holds at least 100 probe array cartridges.

21. The method according to claim 14, wherein:

the cartridge magazine is substantially circular.

22. The method according to claim 14, further comprising:

reducing condensation on at least one of the probe array cartridges by warming.

23. The method according to claim 14, wherein:

the act of transporting comprises one or more elements that reversibly engage and disengage the probe array cartridge.

24. The method according to claim 23, wherein:

the one or more elements engage one or more fiducial features.

25. The apparatus according to claim 1, wherein:

the cartridge transport assembly further comprises reversible rollers capable of transporting probe array cartridges.

26. The apparatus according to claim 1, wherein:

the cartridge transport assembly removes probe array cartridges from the bottom of the cartridge magazine.

27. The apparatus according to claim 1, wherein:

the scanner is capable of fluorescence detection.

28. The apparatus according to claim 1, wherein:

the probe array cartridge is positioned in the cartridge holder based upon information read from the barcode.