Sample processing apparatus with a vision system

• US 7,876,935 B2
• Filed: 01/30/2006
• Issued: 01/25/2011
• Est. Priority Date: 01/30/2006
• Status: Active Grant

• Current Assignee: Protedyne Corporation (Laboratory Corporation Of America Holdings)
• Original Assignee: Protedyne Corporation (Laboratory Corporation Of America Holdings)
• Inventors: Massaro, Peter
• Primary Examiner(s): Mehta; Bhavesh M
• Assistant Examiner(s): Patel; Nirav G
• Application Number:
  US11/342,504
• Publication Number:
  US 20070177778A1
• Time in Patent Office: 1,821 Days
• Field of Search: 382/128
• US Class Current: 382/128
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced

Abstract

A sample processing apparatus has an associated machine vision system. The vision system may acquire an image of at least one fluidic chamber, which can be analyzed by the machine vision system and/or stored. The image and/or analysis of the image may provide a record of the actions of the sample processing apparatus, which permits a user to verify proper operation of the sample processing apparatus.

Claims

1. A method for monitoring a sample handling process, comprising:

associating a liquid sample with a pipette tip;

performing the sample handling process with the liquid sample with respect to the pipette tip, the sample handling process including aspirating or dispensing of the liquid sample with respect to the pipette tip;

using a machine vision system to acquire a 2-dimensional image of at least a portion of the pipette tip before, during or after performance of the sample handling process; and

storing the acquired image to allow verification of a presence or absence of a clot associated with the liquid sample during aspirating or dispensing of the liquid sample.

2. The method of claim 1, wherein the step of associating a liquid sample with a pipette tip includes associating liquid samples with a plurality of pipette tips of a multi-channel pipetting head;

and

the step of using a machine vision system includes acquiring a 2-dimensional image of at least a portion of the plurality of pipette tips.

3. The method of claim 1, further comprising associating the image with an identifier.

4. The method of claim 3, wherein the identifier uniquely identifies the liquid sample.

5. The method of claim 1, further comprising analyzing the image to identify the presence of a clot associated with the pipette tip.

6. The method of claim 5, wherein the analyzing step further includes using the image to determine a volume change of the liquid sample, a presence of an air bubble associated with the liquid sample, or a color associated with the liquid sample.

7. The method of claim 1, wherein the sample handling process comprises dispensing the liquid sample from the pipette tip.

8. A sample handling apparatus, comprising:

at least one pipette tip adapted to hold a liquid sample and allow for aspirating or dispensing of the liquid sample relative to the pipette tip; and

a machine vision system having an image acquisition device positioned to acquire a 2-dimensional image of at least a portion of the pipette tip, the machine vision system being adapted to allow verification of a presence or absence of a clot associated with the pipette tip and the aspiration or dispensing of the liquid sample relative to the at least one pipette tip based on the image.

9. The sample handling apparatus of claim 8, wherein the at least one pipette tip includes a plurality of pipette tips.

10. The sample handling apparatus of claim 8, wherein the machine vision system includes an image analyzer adapted to perform an image analysis operation on the image to verify the presence or absence of a clot associated with the pipette tip.

11. The sample handling apparatus of claim 10, wherein the image analyzer is configured to determine a volume of the liquid sample, an existence of the liquid sample, a presence of an air bubble associated with the liquid sample, a color associated with the liquid sample, and/or a presence of a filter based on the image.

12. The sample handling apparatus of claim 8, wherein the image acquisition device includes a CCD camera.

13. The sample handling apparatus of claim 8, wherein the liquid sample is a blood sample and the image is associated with a source of at least a portion of the liquid sample.

14. The sample handling apparatus of claim 13, wherein the source is a person from whom at least a portion of the liquid sample was taken.

15. The sample handling apparatus of claim 8, wherein the machine vision system is adapted to verify that at least a portion of the liquid sample has been dispensed with respect to the pipette tip based on the image.

16. The sample handling apparatus of claim 8, wherein the machine vision system is adapted to verify that at least a portion of the liquid sample has been filtered.

17. The sample handling apparatus of claim 8, further comprising a robotic system adapted to manipulate the pipette tip with the liquid sample.

18. The sample handling apparatus of claim 8, wherein the vision system is adapted to provide input for control of movement of the robotic system.