Peptide nanostructure-coated electrodes

• US 7,879,212 B2
• Filed: 11/02/2006
• Issued: 02/01/2011
• Est. Priority Date: 11/03/2005
• Status: Active Grant

• Current Assignee: Ramot at Tel Aviv University Limited (Tel Aviv University)
• Original Assignee: Ramot at Tel Aviv University Limited (Tel Aviv University)
• Inventors: Yemini, Miri, Reches, Meital, Rishpon, Judith, Gazit, Ehud
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US11/591,613
• Publication Number:
  US 20070138007A1
• Time in Patent Office: 1,552 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792
• US Class Current: 204/403.01
• CPC Class Codes: C25B 11/075 consisting of a single catalytic element or catalytic compound

Abstract

An electrode coated with peptide nanostructures, composed of self-assembled peptides, is disclosed. The electrode is capable of conducting a response current resulting from an electrochemical reaction. The electrode can form a part of an electrochemical cell, a detector and a sensor array. Methods utilizing an electrochemical cell, a detector or a sensor array comprising the electrode for detecting an Analyte in a sample and kits containing same are also disclosed.

Claims

1. An electrode comprising a plurality of peptide nanostructures, said peptide nanostructures being composed of a plurality of peptides self-assembled into said peptide nano structures, the electrode being capable of conducting a response current resulting from an electrochemical reaction in a proximity thereof, wherein each of said peptides in said plurality of peptides comprises at least one aromatic amino acid residue;

and wherein at least one peptide in said plurality of peptides consists essentially of aromatic amino acid residues.

2. The electrode of claim 1, further comprising a support having a surface, said peptide nanostructures being attached to said surface.

3. The electrode of claim 2, wherein each of said peptide nanostructures is attached to said surface via interactions selected from the group consisting of hydrogen bond interactions, hydrophobic interactions, covalent interactions, coordinative interactions, electrostatic interactions and surface interactions.

4. The electrode of claim 3, wherein at least one peptide in said plurality of peptides forming said peptide nanostructures comprises a functional group for forming said interactions with said surface.

5. The electrode of claim 1, wherein each of said peptides in said plurality of peptides comprises from 2 to 15 amino acid residues.

6. The electrode of claim 1, wherein each of said peptides in said plurality of peptides comprises from 2 to 7 amino acid residues.

7. The electrode of claim 1, wherein at least one peptide in said plurality of peptides is an end-capping modified peptide.

8. The electrode of claim 1, further comprising a moiety being capable of generating an electrochemically reactive molecule and/or capable of reacting with and/or capturing a molecule generating an electrochemically reactive molecule upon said electrochemical reaction.

9. An electrochemical cell comprising a working electrode and a reference electrode, said working electrode being the electrode of claim 8.

10. A process of preparing the electrode of claim 1, the process comprising subjecting said plurality of peptides to conditions which favor formation of said peptide nanostructures.

11. The process of claim 10, wherein said electrode further comprises a support having a surface coated with said peptide nanostructures, the process further comprising:

attaching said peptide nanostructures to said surface.

12. The process of claim 11, further comprising, prior to said attaching:

modifying said peptide nanostructures to thereby generate a functional group thereon, said functional group being for attaching said peptide nanostructures to said surface.

13. The process of claim 10, further comprising, prior to, concomitant with or subsequent to said subjecting, attaching to or encapsulating in said peptide nanostructures a moiety capable of generating an electrochemically reactive molecule and/or capable of reacting with and/or capturing a molecule generating said electrochemically reactive molecule upon said electrochemical reaction.

14. An electrochemical cell comprising a working electrode and a reference electrode, said working electrode being the electrode of claim 1.

15. The electrochemical cell of claim 14, further comprising an auxiliary electrode.

16. The electrochemical cell of claim 14, further comprising an auxiliary electrode.

17. The electrochemical cell of claim 14, further comprising a moiety being capable of generating an electrochemically reactive molecule and/or capable of reacting with and/or capturing a molecule generating said electrochemically reactive molecule upon said electrochemical reaction.

18. The electrochemical cell of claim 17, wherein said moiety is attached to or encapsulated in said peptide nanostructures.

19. A detector comprising:

(a) the electrode of claim 1; and

(b) a detecting unit attached to said electrode and being capable of detecting a response current resulting from said electrochemical reaction.

20. The detector of claim 19, further comprising a reference electrode.

21. The detector of claim 20, further comprising an auxiliary electrode.

22. The detector of claim 19, further comprising a moiety being capable of generating an electrochemically reactive molecule and/or capable of reacting with and/or capturing a molecule generating said electrochemically reactive molecule upon said electrochemical reaction.

23. The detector of claim 22, wherein said moiety forms a part of a first member of a binding pair and said molecule generating said electrochemically reactive molecule forms a part of a second member of a binding pair.

24. The detector of claim 19, wherein said moiety is a ligand, said ligand being capable of capturing a molecule generating said electrochemically active molecule.

25. The detector of claim 24, wherein said ligand is attached to or encapsulated in said peptide nanostructures.

26. A method of electrochemically detecting an analyte is a sample, the method comprising:

contacting the sample with the detector of claim 19; and

measuring said response current.

27. The method of claim 26, wherein the analyte is an electrochemically reactive molecule.

28. The method of claim 26, wherein the analyte forms a part of a first member of a binding pair and said detector further comprises a second member of said binding pair.

29. The method of claim 28, wherein said contacting generates an electrochemically reactive molecule upon interaction between said first and said second members of said binding pairs.

30. The method of claim 28, wherein said second member of said binding pair is attached to or encapsulated in said peptide nanostructures.

31. The method of claim 19, wherein the analyte generates an electrochemically reactive molecule.

32. A kit for detecting an analyte in a sample, the kit comprising the detector of claim 19 being packaged in a packaging material and identified in print, in or on said packaging material, for use in detecting the analyte.

33. The kit of claim 32, wherein the analyte is an electrochemically reactive molecule.

34. The kit of claim 32, wherein the analyte generates an electrochemically reactive moiety.

35. The kit of claim 32, wherein the analyte forms a part of a first member of a binding pair and said detector further comprises a second member of said binding pair.

36. The kit of claim 35, wherein said contacting produces an electrochemically reactive molecule upon interaction between said first and said second members of said binding pairs.

37. The kit of claim 35, wherein said second member of said binding pair is attached to or encapsulated in said peptide nanostructures.

38. The kit of claim 35, wherein said detector and said second member of said binding pair are individually packaged within the kit.

39. A detector comprising:

(a) the electrode of claim 1;

(b) a detecting unit attached to said electrode and being capable of detecting a response current resulting from said electrochemical reaction; and

(c) a moiety being capable of generating an electrochemically reactive molecule and/or capable of reacting with and/or capturing a molecule generating said electrochemically reactive molecule upon said electrochemical reaction, wherein said moiety is an enzyme, said enzyme being capable of catalyzing a reaction generating said electrochemically reactive molecule.

40. The detector of claim 39, wherein said enzyme is attached to or encapsulated in said peptide nano structures.

41. A sensor array comprising a plurality of electrochemical cells each comprising, as a working electrode, the electrode of claim 1.

42. The sensor array of claim 41, wherein each of said plurality of electrochemical cells further includes a reference electrode.

43. The sensor array of claim 42, wherein each of said plurality of electrochemical cells further includes an auxiliary electrode.

44. The sensor array of claim 41, wherein the sensor array includes a support having a plurality of chambers, and whereas each of said plurality of electrochemical cells is disposed within a specific chamber of said plurality of chambers.

45. An electrode comprising a plurality of peptide nanostructures, said peptide nanostructures being composed of a plurality of peptides self-assembled into said peptide nano structures, the electrode being capable of conducting a response current resulting from an electrochemical reaction in a proximity thereof, wherein each of said peptides in said plurality of peptides comprises from 2 to 15 amino acid residues;

and wherein at least one peptide in said plurality of peptides is a dipeptide.

46. The electrode of claim 45, wherein each peptide in said plurality of peptides is a phenylalanine-phenylalanine dipeptide.

47. An electrode comprising a plurality of peptide nanostructures, said peptide nanostructures being composed of a plurality of peptides self-assembled into said peptide nano structures, the electrode being capable of conducting a response current resulting from an electrochemical reaction in a proximity thereof, the electrode comprising a moiety being capable of generating an electrochemically reactive molecule and/or capable of reacting with and/or capturing a molecule generating an electrochemically reactive molecule upon said electrochemical reaction;

wherein said moiety is an enzyme, said enzyme being capable of catalyzing a reaction generating said electrochemically reactive molecule.

48. An electrode comprising a plurality of peptide nanostructures, said peptide nanostructures being composed of a plurality of peptides self-assembled into said peptide nano structures, the electrode being capable of conducting a response current resulting from an electrochemical reaction in a proximity thereof, the electrode comprising a moiety being capable of generating an electrochemically reactive molecule and/or capable of reacting with and/or capturing a molecule generating an electrochemically reactive molecule upon said electrochemical reaction;

wherein said moiety is a ligand, said ligand being capable of capturing said molecule generating said electrochemically reactive molecule.