Apparatus and methods of detecting features on a microarray

• US 7,879,541 B2
• Filed: 04/24/2003
• Issued: 02/01/2011
• Est. Priority Date: 10/26/2000
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Kincaid, Robert H.
• Primary Examiner(s): CROW, ROBERT THOMAS
• Application Number:
  US10/422,163
• Publication Number:
  US 20030186310A1
• Time in Patent Office: 2,840 Days
• Field of Search: 435/6, 435/287.1, 435/287.2, 436/46, 422/55
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: Y10T 436/112499 with sample on test slide

Abstract

A method of making a microarray apparatus with enhanced feature detectability provides for accurate detection of each feature location, regardless of the quality or quantity of signals from hybridized oligomer test probes. The method comprises separately providing a control probe or stilt and an oligomer test probe at each feature location on the microarray, such that each feature comprises a control probe and a test probe. The control probe comprises a sequence of nucleic acids unique to the control probe. The control probe is labeled with a label that emits a control signal. The oligomer test probe is labeled with a test label that emits a test signal distinguishable from the control signal. When the microarray is hybridized and interrogated, the control signal indicates the location of each and every feature on the array and the test signal indicates the location of hybridized oligomer test probes.

Claims

1. A method of performing a nondestructive quality control evaluation of a microarray comprising:

providing a labeled control probe to a plurality of feature locations on the surface of a microarray substrate, the labeled control probe on the microarray emitting a control probe signal when excited by a light;

locating each feature location of said plurality of feature locations by detecting the control probe signal at each feature location of said plurality of feature locations, wherein said locating comprises interrogating the microarray;

evaluating data acquired from the interrogation, wherein evaluating comprises using the data acquired for modifying a subsequent deposition; and

subsequently depositing an oligomer test probe to each of said feature locations of the microarray based on the acquired data, such that a feature comprising the labeled control probe and the oligomer test probe is provided at each feature location.

2. The method of claim 1, further comprising after subsequently depositing:

forwarding the data acquired to a user of the microarray to assist the user in evaluating assay results of a hybridization assay subsequently performed by the user.

3. The method of claim 2, further comprising:

subsequently performing the hybridization assay on the microarray with a labeled test target using the acquired data, such that hybridized oligomer test probes are produced on one or more features, the hybridized oligomer test probes being indirectly associated with a test target label that emits a test target signal when excited by the light, the test target signal being different from the control probe signal.

4. The method of claim 2, further comprising interrogating the microarray after subsequently performing the hybridization assay to obtain the assay results, wherein interrogating comprises:

scanning the microarray with a microarray scanner to excite the labels;

collecting the control probe signal in a control detection channel; and

collecting the test target signal in a test detection channel that is separate from the control detection channel of a detection system of the microarray scanner,

wherein the separately collected signals facilitate evaluation of the assay results.

5. The method of claim 2, wherein the oligomer test probe comprises a test probe label that emits a test probe signal when excited by a light, the test probe signal being different from the control probe signal and the test target signal.

6. The method of claim 5, further comprising interrogating the microarray after subsequently performing the hybridization assay to obtain the assay results, wherein interrogating comprises:

scanning the microarray with a microarray scanner to excite the labels;

collecting the control probe signal in a control detection channel;

collecting the test probe signal in a first test detection channel; and

collecting the test target signal in a second test detection channel that is separate from the control detection channel and the first test detection channel of a detection system of the microarray scanner,

wherein the separately collected signals facilitate evaluation of the assay results.

7. The method of claim 1, further comprising providing the microarray, a control target and the acquired data to a user, the control target being specifically complementary to the control probe, the control target comprising a control target label that emits a control target signal when excited by light, the control target signal being different from the control probe signal and the test target signal, and

subsequently performing a hybridization assay on the microarray with the control target and a test target, the test target comprising a test target label that emits a test target signal when excited by light, each of the test target signal and the control target signal being different from each other and being different from the control probe signal.

8. The method of claim 7, further comprising interrogating the microarray after subsequently performing the hybridization assay to obtain the assay results, wherein interrogating comprises:

scanning the microarray with a microarray scanner to excite the labels;

collecting the control probe signal in a first control detection channel;

collecting the control target signal in a second control detection channel that is separate from the first control detection channel; and

collecting the test target signal in a second test detection channel that is separate from the first test detection channel and the control detection channels,

wherein the separately collected signals facilitate evaluation of the assay results.

9. The method of claim 7, wherein the oligomer test probe comprises a test probe label that emits a test probe signal when excited by a light, the test probe signal being different from each of the control probe signal, the control target signal and the test target signal.

10. The method of claim 9, further comprising interrogating the microarray after subsequently performing the hybridization assay to obtain the assay results, wherein interrogating comprises:

scanning the microarray with a microarray scanner to excite the labels;

collecting the control probe signal in a first control detection channel;

collecting the control target signal in a second control detection channel that is separate from the first control detection channel;

collecting the test probe signal in a first test detection channel separate from the control detection channels; and

collecting the test target signal in a second test detection channel that is separate from the first test detection channel and the control detection channels,

wherein the separately collected signals facilitate evaluation of the assay results.