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Nucleic acid amplification and detection of mycobacterium species

  • US 7,879,581 B2
  • Filed: 11/12/2007
  • Issued: 02/01/2011
  • Est. Priority Date: 12/17/1999
  • Status: Expired due to Fees
First Claim
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1. A method of detecting Mycobacterium species present in a biological sample, comprising the steps of:

  • providing a biological sample containing nucleic acid from at least one Mycobacterium species comprising a Mycobacterium 16S ribosomal RNA (rRNA) or DNA encoding a Mycobacterium 16S rRNA;

    amplifying the Mycobacterium 16S rRNA or Mycobacterium DNA encoding the Mycobacterium 16S rRNA in an in vitro nucleic acid amplification mixture comprising at least one polymerase activity, and a combination of at least one first oligonucleotide and at least one second oligonucleotide, wherein the first oligonucleotide consists a target-specific sequence that consists of SEQ ID NO;

    5 joined to a 5′

    promoter sequence or SEQ ID NO;

    11, and the second oligonucleotide consists of SEQ ID NO;

    16 or SEQ ID NO;

    38 to produce amplified Mycobacterium nucleic acid; and

    detecting the amplified Mycobacterium nucleic acid by detecting a label associated with the amplified Mycobacterium nucleic acid.

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