Production of cell culture product and material for use in said production

• US 7,883,865 B2
• Filed: 09/08/2005
• Issued: 02/08/2011
• Est. Priority Date: 09/08/2004
• Status: Expired due to Fees

• Current Assignee: Nagoya University
• Original Assignee: Nagoya University
• Inventors: Takai, Osamu, Honda, Hiroyuki, Ito, Akira, Wu, Yunying, Saito, Nagahiro
• Primary Examiner(s): Ketter; James S
• Application Number:
  US11/574,949
• Publication Number:
  US 20080032403A1
• Time in Patent Office: 1,979 Days
• Field of Search: None
• US Class Current: 435/69.1
• CPC Class Codes: A61L 2430/36 for embolization or occlusion, e.g. vaso-occlusive compositions or devices artificial blood vessels A61L27/507
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12M 25/01 Drops
• CPC Class Codes: C12M 25/06 Plates; Walls; Drawers; Multilayer plates
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 2535/10 Patterned coating
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/069 Vascular Endothelial cells

Abstract

A base plate for use in cell culture which comprises a base material, a water-repellent layer having a water-repellent surface formed on the above base material and a hydrophilic surface having a prescribed pattern formed on the base material; a method for producing the above base plate; and a method for producing a cell culture product using the base plate. It is preferred that the water-repellent surface has a water contact angle of more than 150°. The above method for producing a cell culture product using the base plate base plate is a novel method which allows the production of a cell culture product of a fine pattern.

Claims

1. A method for producing a cell culture product in a desired pattern, the method comprising:

preparing a plate for cell culture, the plate having a hydrophilic surface formed in a groove of a width of less than or equal to 100 μ

m and a water repellent surface having a water contact angle of greater than 150°

formed in areas other than the groove;

disposing protruding droplets of a culture medium selectively along the groove on the hydrophilic surface; and

culturing a target cell in the disposed medium.

2. The method of claim 1, wherein the water repellent surface is formed of a high molecular weight organic compound having an alkyl, alkenyl, or alkynyl group substituted or not substituted with a functional group capable of bonding to the plate.

3. The method of claim 2, wherein the high molecular weight organic compound is an organic compound having a functional group capable of bonding to the plate and an alkyl, alkenyl or alkynyl group substituted entirely or partially with fluorine.

4. The method of claim 1, wherein the water repellent surface is formed of a monomolecular layer.

5. The method of claim 1, wherein the water repellent surface is formed of a polysiloxane film with fluorine substituted alkyl side chains.

6. The method of claim 1, wherein the groove is formed by removing a portion of the water repellent layer from the plate by lithography.

7. The method of claim 1, wherein the cell is an embryonic stem cell or a bone marrow stem cell.

8. A method for producing a cell culture product in a desired pattern, the method comprising:

preparing a plate for cell culture, the plate having a hydrophilic surface formed in a groove of a width of less than or equal to 100 μ

m and a water repellent surface having a water contact angle of greater than 150°

formed in areas other than the groove; and

culturing a target adherent cell in a state in which the cells are adhered selectively to the hydrophilic surface, wherein protruding droplets of a culture medium are disposed along the groove of the hydrophilic surface.

9. The method of claim 8, wherein the water repellent surface is formed of a high molecular weight organic compound having an alkyl, alkenyl, or alkynyl group substituted or not substituted with a functional group capable of bonding to the plate.

10. The method of claim 9, wherein the high molecular weight organic compound is an organic compound having a functional group capable of bonding to the plate and an alkyl, alkenyl or alkynyl group substituted entirely or partially with fluorine.

11. The method of claim 8, wherein the water repellent surface is formed of a monomolecular layer.

12. The method of claim 8, wherein the water repellent surface is formed of a polysiloxane film with fluorine substituted alkyl side chains.

13. The method of claim 8, wherein the groove is formed by removing a portion of the water repellent layer from the plate by lithography.

14. The method of claim 8, wherein the adherent cell is one selected from the group consisting of a vascular endothelium cell, a neural cell, a fibroblast, a hepatocyte, and an osteoblast.

15. The method of claim 8, wherein the cell culture product is a capillary network.