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Dendritic chemiluminescent substrates

  • US 7,883,903 B2
  • Filed: 04/03/2007
  • Issued: 02/08/2011
  • Est. Priority Date: 01/08/2001
  • Status: Active Grant
First Claim
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1. A kit for conducting an assay to determine the presence or concentration of an analyte in a sample, wherein the kit comprises:

  • a dendrimer comprising at least one enzymatically active chemiluminescent substrate moiety, wherein the substrate moiety comprises a dioxetane having an enzyme labile group; and

    an enzyme capable of cleaving the enzyme labile group of the dioxetane to produce a peroxygenated intermediate that decomposes to produce light;

    wherein the dendrimer comprising at least one enzymatically active chemiluminescent substrate moiety is represented by the formula selected from the group consisting of

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