Analyte sensors and methods of use

• US 7,887,682 B2
• Filed: 03/29/2006
• Issued: 02/15/2011
• Est. Priority Date: 03/29/2006
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Watkin, Jared L., Wang, Yi, Feldman, Benjamin J.
• Primary Examiner(s): Neckel; Alexa D
• Assistant Examiner(s): Dieterle; Jennifer
• Application Number:
  US11/277,931
• Publication Number:
  US 20070227911A1
• Time in Patent Office: 1,784 Days
• Field of Search: 204/403.1, 204/403.01, 204/403.14, 204/403.12, 204/409, 204/411, 204/412, 205/792, 205/775, 205/779, 205/777.5, 422/82.01
• US Class Current: 204/403.01
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10T 436/200833 Carbonyl, ether, aldehyde or ketone containing

Abstract

In vitro analyte sensors and methods of analyte determination are provided. Embodiments include sensors that include a pair of electrodes to monitor filling of the sample chamber with sample.

Claims

1. A sensor for determining the concentration of an analyte in a sample, the sensor comprising:

a sample chamber;

a first electrode pair, wherein electrodes of the first electrode pair are spaced in a lateral direction with respect to each other in the sample chamber, and

a second electrode pair, wherein electrodes of the second electrode pair each comprise an electrode portion and a trace portion and wherein entire electrode portions of the electrodes of the second electrode pair are spaced in a longitudinal direction with respect to each other in the sample chamber,

wherein the second electrode pair is positioned in between the first electrode pair.

2. The sensor of claim 1, wherein the electrode portions of the electrodes of the second electrode pair are substantially rectangular.

3. The sensor of claim 1, wherein the sensor further comprises a first substrate, a second substrate, and a sample receiving end, wherein the sample chamber is positioned between the first and second substrates, wherein the sensor further comprises an overhanging substrate wall at the sample receiving end of the sensor, and wherein the sample chamber comprises at least two unbounded sides at the sample receiving end of the sensor.

4. The sensor of claim 1, wherein the first electrode pair comprises a pair of electrodes that functions as indicator electrodes and counter electrodes and the second electrode pair is a pair is a pair of working electrodes.

5. The sensor of claim 1, wherein the second electrode pair comprises a pair of electrodes that functions as indicator electrodes and counter electrodes and the first electrode pair is a pair is a pair of working electrodes.

6. The sensor of claim 1, wherein the second electrode pair comprises a pair of working electrodes.

7. The sensor of claim 1, wherein the second electrode pair comprises a pair of counter electrodes.

8. The sensor of claim 1, wherein about 10% to about 95% of perimeter of the sample chamber is unbounded.

9. The sensor of claim 8, wherein about 50% to about 95% of perimeter of the sample chamber is unbounded.

10. The sensor of claim 1, wherein the sample chamber comprises at least two unbounded sides.

11. The sensor of claim 10, wherein the sample chamber comprises three unbounded sides.

12. The sensor of claim 1, wherein the sample chamber has a volume of no more than about 1 microliter.

13. The sensor of claim 12, wherein the sample chamber has a volume of no more than about 0.5 microliter.

14. The sensor of claim 13, wherein the sample chamber has a volume of no more than about 0.1 microliter.

15. The sensor of claim 1, wherein the sensor further comprises a first substrate and a second substrate, a spacer between the first substrate and the second substrate, together the first substrate, second substrate and spacer defining at least a portion of the sample chamber, wherein the first electrode pair and the second electrode pair are placed on the first substrate.

16. The sensor of claim 15, wherein the first electrode pair comprises a pair of indicator electrodes.

17. The sensor of claim 16, wherein the second electrode pair comprises a pair of counter electrodes.

18. The sensor of claim 17, wherein the sample chamber further comprises a working electrode placed on the second substrate.