Methods of making enhanced, autologous fat grafts
First Claim
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1. A method of making an enhanced, autologous fat graft comprising:
- extracting from a subject a first portion of adipose tissue to be used as a fat graft, thensupplementing said fat graft with an additive and a population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells, wherein said stem cells and endothelial precursor cells are prepared by disaggregating and concentrating the stem cells and endothelial precursor cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.
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Abstract
Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.
95 Citations
20 Claims
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1. A method of making an enhanced, autologous fat graft comprising:
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extracting from a subject a first portion of adipose tissue to be used as a fat graft, then supplementing said fat graft with an additive and a population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells, wherein said stem cells and endothelial precursor cells are prepared by disaggregating and concentrating the stem cells and endothelial precursor cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method of making an enhanced, autologous fat graft comprising:
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(a) disaggregating a first portion of extracted adipose tissue with an enzyme or mechanical force to generate a cell suspension; (b) concentrating the cells in said cell suspension by buoyant density sedimentation, filtration or centrifugation to obtain a population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells; (c) supplementing said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells with an additive; (d) supplementing a second portion of extracted adipose tissue to be used as a fat graft with the population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells, so as to make said enhanced, autologous fat graft. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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20. A method of making an enhanced, autologous fat graft, comprising extracting from a subject a first portion of adipose tissue to be used as a fat graft, then supplementing said fat graft with a population of concentrated, disaggregated adipose-derived stem cells and an additive, wherein the stem cells are prepared by disaggregating and concentrating the stem cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.
Specification