Modified green fluorescent proteins and methods for using same

• US 7,888,113 B2
• Filed: 07/24/2008
• Issued: 02/15/2011
• Est. Priority Date: 11/04/2005
• Status: Active Grant

• Current Assignee: Evrogen Joint Stock Company
• Original Assignee: Evrogen Joint Stock Company
• Inventors: Lukyanov, Sergey A.
• Primary Examiner(s): Monshipouri; Maryam
• Application Number:
  US12/179,000
• Publication Number:
  US 20090117650A1
• Time in Patent Office: 936 Days
• Field of Search: 536/23.1, 435/252.3, 435/320.1
• US Class Current: 435/320.1
• CPC Class Codes: C07K 14/43595 from coelenteratae, e.g. medusae

Abstract

The present invention provides nucleic acid molecules encoding mutant fluorescent proteins as well as proteins encoded by these nucleic acids. In addition, host-cells, stable cell lines and transgenic organisms comprising the above-referenced nucleic acid molecules are provided. The subject protein and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cells, or cell organelles.

Claims

1. An isolated nucleic acid, comprising a nucleic acid sequence encoding a genetically engineered fluorescent protein that has at least 95% identity with the amino acid sequence of SEQ ID NO:

22.

2. An isolated nucleic acid of claim 1, wherein the genetically engineered fluorescent protein has at least 97% identity with the amino acid sequence of SEQ ID NO:

22.

3. The nucleic acid of claim 1, wherein the genetically engineered fluorescent protein has the amino acid sequence of SEQ ID NO:

22.

4. A vector comprising the nucleic acid of claim 1.

5. An expression cassette comprising:

(a) a transcriptional initiation region that is functional in an expression host;

(b) the nucleic acid according to claim 1; and

(c) and a transcriptional termination region functional in said expression host.

6. An host cell or progeny thereof, comprising the expression cassette according to claim 5 as part of an extrachromosomal element or integrated into the genome of a host cell as a result of introduction of said expression cassette into said host cell.

7. A transgenic cell, or progeny thereof, comprising the nucleic acid according to claim 1.

8. A kit comprising at least one nucleic acid according to claim 1.