Chemokine-binding protein and methods of use
First Claim
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1. A method of identifying a chemokine binding domain having an altered specificity or affinity, said method comprising the steps of:
- (a) altering the nucleotide sequence of a nucleic acid comprising a polynucleotide sequence that encodes a chemokine binding domain of the amino acid sequence 143-213 of SEQ ID NO;
3, thereby generating an altered chemokine binding domain;
(b) determining whether the specificity or chemokine binding affinity of said altered chemokine binding domain is changed as compared to the chemokine binding domain prior to the alteration;
(c) optionally, repeating steps (a) and (b); and
(d) selecting the desired chemokine binding domain having an altered specificity or affinity.
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Abstract
Described herein are chemokine-binding domains of THAP-family polypeptides and pharmaceutical compositions which include a polypeptide comprising a chemokine-binding domain of a THAP-family polypeptide. Also disclosed are methods of binding chemokines, inhibiting the activity of chemokines, detecting chemokines, and reducing the symptoms associated with a chemokine mediated or influenced condition by contacting the chemokine with an agent that includes a polypeptide comprising a chemokine binding domain of a THAP-family polypeptide.
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Citations
12 Claims
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1. A method of identifying a chemokine binding domain having an altered specificity or affinity, said method comprising the steps of:
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(a) altering the nucleotide sequence of a nucleic acid comprising a polynucleotide sequence that encodes a chemokine binding domain of the amino acid sequence 143-213 of SEQ ID NO;
3, thereby generating an altered chemokine binding domain;(b) determining whether the specificity or chemokine binding affinity of said altered chemokine binding domain is changed as compared to the chemokine binding domain prior to the alteration; (c) optionally, repeating steps (a) and (b); and (d) selecting the desired chemokine binding domain having an altered specificity or affinity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification