Mesoscale polynucleotide amplification devices
First Claim
1. A device for amplifying a polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising a solid substrate microfabricated to define:
- a sample inlet port;
a mesoscale flow system comprising;
a sample flow channel extending from said inlet port; and
a polynucleotide amplification region of the device, in fluid communication with said flow channel, said region having at least one cross-sectional dimension of width or depth of about 0.1 to 1000 microns, at least said polynucleotide amplification region being permanently sealed with a cover; and
an appliance for use in combination with said substrate, said appliance including a nesting site for receiving said substrate and a conduit in fluid communication with the inlet port of said substrate.
0 Assignments
0 Petitions
Accused Products
Abstract
Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction, chamber, having at least one cross-sectional dimension of about 0.1 to 1000 μm. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation.
-
Citations
16 Claims
-
1. A device for amplifying a polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising a solid substrate microfabricated to define:
-
a sample inlet port; a mesoscale flow system comprising; a sample flow channel extending from said inlet port; and a polynucleotide amplification region of the device, in fluid communication with said flow channel, said region having at least one cross-sectional dimension of width or depth of about 0.1 to 1000 microns, at least said polynucleotide amplification region being permanently sealed with a cover; and an appliance for use in combination with said substrate, said appliance including a nesting site for receiving said substrate and a conduit in fluid communication with the inlet port of said substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
-
13. A method for amplifying a polynucleotide in a sample by conducting a polynucleotide amplification reaction, the method comprising:
-
(i) providing a device comprising a solid substrate microfabricated to define; a sample inlet port; a mesoscale flow system comprising; a sample flow channel extending from said inlet port; and a polynucleotide amplification region, in fluid communication with said flow channel, said region having at least one cross sectional dimension of width or depth of about 0.1 to 1000 microns, at least said polynucleotide amplification region being permanently sealed with a cover; and an appliance for use in combination with said substrate, said appliance including a nesting site for receiving said substrate and a conduit in fluid communication with the inlet port of said substrate; (ii) delivering through said conduit, said inlet port and said sample flow channel to said polynucleotide amplification region, said sample polynucleotide and at least one reagent required for the polynucleotide amplification reaction; and (iii) thermally controlling the contents of the amplification region to amplify said polynucleotide. - View Dependent Claims (14, 15, 16)
-
Specification