Pluripotent stem cell growing method
First Claim
1. A method for growing pluripotent stem cells which exhibit a normal karyotype comprising contacting said pluripotent stem cells with a liquid medium and growing said pluripotent stem cells in said liquid medium in a dispersed state in a culturing vessel without using feeder cells, wherein said pluripotent stem cells maintain their undifferentiated state and pluripotency, said culturing vessel including immobilized or coated on a substrate solid phase surface a cadherin molecule, and wherein said pluripotent stem cells achieve cell counts at least two times greater than said pluripotent stem cells cultured on a gelatin plate after four days.
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Abstract
A novel growing method is provided for pluripotent stem cells such as ES cells. The method of the invention is a pluripotent stem cell growing method and gene transfer method in which pluripotent stem cells are cultured under conditions that maintain their undifferentiated state and pluripotency, the method being characterized by using a liquid medium and a culturing vessel having immobilized or coated on a substrate solid phase surface a molecule which is adhesive to the pluripotent stem cells in a fixed concentration, to grow the pluripotent stem cells in a dispersed state while maintaining their undifferentiated state and pluripotency, without using feeder cells, or to transfer and express a gene therein.
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Citations
13 Claims
- 1. A method for growing pluripotent stem cells which exhibit a normal karyotype comprising contacting said pluripotent stem cells with a liquid medium and growing said pluripotent stem cells in said liquid medium in a dispersed state in a culturing vessel without using feeder cells, wherein said pluripotent stem cells maintain their undifferentiated state and pluripotency, said culturing vessel including immobilized or coated on a substrate solid phase surface a cadherin molecule, and wherein said pluripotent stem cells achieve cell counts at least two times greater than said pluripotent stem cells cultured on a gelatin plate after four days.
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