Articles of manufacture for detection of herpes simplex virus
First Claim
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1. An article of manufacture comprising:
- a pair of HSV TK primers, wherein said pair of HSV TK primers comprise a first HSV TK primer and a second HSV TK primer, wherein said first HSV TK primer consists of the sequence 5′
-CAC GCT RCT GCG GGT TTA TAT AGA-3′
(SEQ ID NO;
7), wherein R is A or G, and wherein said second HSV TK primer consists of the sequence 5′
-TTG TTA TCT GGG CGC TMG TCA TT-3′
(SEQ ID NO;
8), wherein M is A or C;
a pair of HSV TK probes, wherein said pair of HSV TK probes comprise a first HSV TK probe and a second HSV TK probe, wherein said first HSV TK probe consists of the sequence 5′
-CGC GCG ACG ATA TCG TCT ACG TAC-3′
(SEQ ID NO;
9), and wherein said second HSV TK probe consists of the sequence 5′
-CGA GCC GAT GAC TTA CTG GCA GGT G-3′
(SEQ ID NO;
10); and
a donor fluorescent moiety and a corresponding acceptor moiety.
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Abstract
The invention provides methods to detect herpes simplex virus (HSV) in biological samples and further to distinguish between HSV-1 and HSV-2. Primers and probes for the differential detection of HSV-1 and HSV-2 are provided by the invention. Articles of manufacture containing such primers and probes for detecting HSV are further provided by the invention.
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4 Claims
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1. An article of manufacture comprising:
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a pair of HSV TK primers, wherein said pair of HSV TK primers comprise a first HSV TK primer and a second HSV TK primer, wherein said first HSV TK primer consists of the sequence 5′
-CAC GCT RCT GCG GGT TTA TAT AGA-3′
(SEQ ID NO;
7), wherein R is A or G, and wherein said second HSV TK primer consists of the sequence 5′
-TTG TTA TCT GGG CGC TMG TCA TT-3′
(SEQ ID NO;
8), wherein M is A or C;a pair of HSV TK probes, wherein said pair of HSV TK probes comprise a first HSV TK probe and a second HSV TK probe, wherein said first HSV TK probe consists of the sequence 5′
-CGC GCG ACG ATA TCG TCT ACG TAC-3′
(SEQ ID NO;
9), and wherein said second HSV TK probe consists of the sequence 5′
-CGA GCC GAT GAC TTA CTG GCA GGT G-3′
(SEQ ID NO;
10); anda donor fluorescent moiety and a corresponding acceptor moiety. - View Dependent Claims (2, 3, 4)
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Specification