Stabilizing agents and capture ligands for use in assays measuring analyte concentrations
First Claim
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1. A method for measuring a concentration of a free analyte, wherein analyte exists in an equilibrium between free analyte and bound analyte in a sample, the method comprising:
- (a) forming a test mixture by adding to a vessel;
(i) a capture ligand for the free analyte,(ii) 2-ethyl-hexyl sulfate, or salt thereof in an amount effective to preserve an equilibrium between free analyte and bound analyte, and(iii) the sample comprising the analyte;
(b) adding a detection system to the vessel; and
(c) measuring the concentration of the analyte in the sample using the detection system.
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Abstract
The present invention is related to compositions useful for the measurement of free or unbound analyte concentrations in a fluid. The present invention includes the use of capture ligands and stabilizing agents to improve the accuracy of analyte concentration assays. Methods and tools for using the present invention are also disclosed.
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Citations
12 Claims
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1. A method for measuring a concentration of a free analyte, wherein analyte exists in an equilibrium between free analyte and bound analyte in a sample, the method comprising:
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(a) forming a test mixture by adding to a vessel; (i) a capture ligand for the free analyte, (ii) 2-ethyl-hexyl sulfate, or salt thereof in an amount effective to preserve an equilibrium between free analyte and bound analyte, and (iii) the sample comprising the analyte; (b) adding a detection system to the vessel; and (c) measuring the concentration of the analyte in the sample using the detection system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification