Direct differentiation method for making cardiomyocytes from human embryonic stem cells
First Claim
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1. A method of differentiating a primate pluripotent stem (pPS) cell into a cell expressing α
- -MHC comprising a) plating the undifferentiated pPS cell without forming an embryoid body directly onto a solid surface comprising a substrate;
b) contacting the pPS cell plated on the solid surface with one of the following;
1) BMP;
or
2) activin and BMP;
c) culturing the contacted pPS cell of b), thereby directing differentiation of the pPS cell into a cell expressing α
-MHC.
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Abstract
This invention provides a new procedure for generating cardiomyocyte lineage cells from embryonic stem cells for use in regenerative medicine. Differentiating by way of embryoid body formation or in serum is no longer required. Instead, the stem cells are plated onto a solid substrate, and differentiated in the presence of select factors and morphogens. After enrichment for cells with the appropriate phenotype, the cells are allowed to cluster into Cardiac Bodies™, which are remarkably homogeneous and suitable for the treatment of heart disease.
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Citations
14 Claims
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1. A method of differentiating a primate pluripotent stem (pPS) cell into a cell expressing α
- -MHC comprising a) plating the undifferentiated pPS cell without forming an embryoid body directly onto a solid surface comprising a substrate;
b) contacting the pPS cell plated on the solid surface with one of the following;
1) BMP;
or
2) activin and BMP;
c) culturing the contacted pPS cell of b), thereby directing differentiation of the pPS cell into a cell expressing α
-MHC. - View Dependent Claims (2, 3, 4, 5, 6)
- -MHC comprising a) plating the undifferentiated pPS cell without forming an embryoid body directly onto a solid surface comprising a substrate;
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7. A method of differentiating a primate pluripotent stem (pPS) cell into a cell expressing Nkx2.5 comprising a) plating the undifferentiated pPS cell without forming an embryoid body directly onto a solid surface comprising a substrate;
- b) contacting the pPS cell with activin and BMP;
c) culturing the contacted pPS cell of b), thereby directing differentiation of the pPS cell into a cell expressing Nkx2.5. - View Dependent Claims (8, 9, 10)
- b) contacting the pPS cell with activin and BMP;
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11. A method of differentiating a primate pluripotent stem (pPS) cell into a cell expressing α
- -sarcomeric actinin comprising a) plating the undifferentiated pPS cell without forming an embryoid body directly onto a solid surface comprising a substrate;
b) contacting the pPS cell with activin and BMP;
c) culturing the contacted pPS cell of b), thereby directing differentiation of the pPS cell into a cell expressing α
-sarcomeric actinin. - View Dependent Claims (12, 13, 14)
- -sarcomeric actinin comprising a) plating the undifferentiated pPS cell without forming an embryoid body directly onto a solid surface comprising a substrate;
Specification