Conjugated polymers suitable for strand-specific polynucleotide detection in homogeneous and solid state assays
First Claim
1. A conjugated polymer that can interact with a biomolecule, said polymer soluble in a polar medium,wherein said polymer comprises a plurality of low bandgap repeat units sufficient for the polymer to form an excited state when the polymer is contacted with incident light in the region of about 450 nm to about 1000 nm in the absence of the target biomolecule, and wherein the polymer can transfer energy from its excited state to a fluorophore to provide a greater than 50% increase in fluorescence emission from the fluorophore than can be achieved by direct excitation.
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Abstract
The invention further relates to polycationic multichromophores, which may be conjugated polymers, and methods, articles and compositions employing them as described herein. In some aspects, the invention relates to methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence of a target biomolecule in a sample or its relative amount, or the assays may be quantitative or semi-quantitative. The methods can be performed on a substrate. The methods can be performed in an array format on a substrate, which can be a sensor. In some embodiments, detection assays are provided employing sensor biomolecules that do not comprise a fluorophore that can exchange energy with the cationic multichromophore. In some aspects biological assays are provided in which energy is transferred between one or more of the multichromophore, a label on the target biomolecule, a label on the sensor biomolecule, and/or a fluorescent dye specific for a polynucleotide, in all permutations. The multichromophore may interact at least in part electrostatically with the sensor and/or the target, and an increase in energy transfer with the polymer may occur upon binding of the sensor and the target. Other variations of the inventions are described further herein.
84 Citations
61 Claims
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1. A conjugated polymer that can interact with a biomolecule, said polymer soluble in a polar medium,
wherein said polymer comprises a plurality of low bandgap repeat units sufficient for the polymer to form an excited state when the polymer is contacted with incident light in the region of about 450 nm to about 1000 nm in the absence of the target biomolecule, and wherein the polymer can transfer energy from its excited state to a fluorophore to provide a greater than 50% increase in fluorescence emission from the fluorophore than can be achieved by direct excitation.
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31. An aqueous solution comprising a conjugated polymer that can interact with a biomolecule
wherein said polymer comprises a plurality of low bandgap repeat units sufficient for the polymer to form an excited state when the polymer is contacted with incident light in the region of about 450 nm to about 1000 nm in the absence of the target biomolecule.
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32. A polymer that can interact with a target biomolecule, the polymer being soluble in a polar medium and wherein the polymer and the target interact,
wherein the polymer comprises a plurality of low bandgap repeat units sufficient for the polymer to form an excited state when the polymer is contacted with incident light in the region of about 450 nm to about 1000 nm in the absence of the target biomolecule, and wherein the polymer can transfer energy from its excited state to a fluorophore, the fluorophore generating more fluorescence with the polymer present than if the polymer is not present.
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46. A method for detecting a target biomolecule, said method comprising adding a conjugated polymer capable of interacting with a target biomolecule to a medium containing a mixture of biomolecules,
wherein said polymer is soluble in a polar medium and comprises a plurality of low bandgap repeat units sufficient for the polymer to form an excited state when the polymer is contacted with incident light in the region of about 450 nm to about 1000 nm in the absence of the target biomolecule, and wherein the polymer can transfer energy from its excited state to a fluorophore to provide a greater than 50% increase in fluorescence emission from the fluorophore than can be achieved by direct excitation.
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51. A method for detecting a target biomolecule, said method comprising adding a conjugated polymer capable of interacting with a target biomolecule to a medium containing a mixture of biomolecules,
wherein said polymer is soluble in a polar medium and comprises a plurality of low bandgap repeat units sufficient for the polymer to form an excited state when the polymer is contacted with incident light in the region of about 450 nm to about 1000 nm in the absence of the target biomolecule, and wherein the polymer can transfer energy from its excited state to a fluorophore, the fluorophore generating more fluorescence with the polymer present than if the polymer is not present.
- 56. A method of performing fluorescence energy resonance transfer (FRET) comprising (a) adding a combination of a conjugated polymer, a sensor biomolecule and a fluorophore and (b) detecting emission form the fluorophore by exciting the conjugated polymer, wherein said polymer is soluble in a polar medium and comprises a plurality of low bandgap repeat units sufficient for the polymer to form an excited state when the polymer is contacted with incident light in the region of about 450 nm to about 1000 nm in the absence of the target biomolecule, and wherein the polymer can transfer energy from its excited state to a fluorophore, the fluorophore generating more fluorescence with the polymer present than if the polymer is not present.
Specification