Barley Lipoxygenase 1 Gene, method of selecting barley variety, material of malt alcoholic drinks and process for producing malt alcoholic drink

• US 7,897,850 B2
• Filed: 03/25/2004
• Issued: 03/01/2011
• Est. Priority Date: 03/25/2003
• Status: Expired due to Fees

• Current Assignee: Sapporo Breweries Limited (Sapporo Holdings Limited)
• Original Assignee: Sapporo Breweries Limited (Sapporo Holdings Limited)
• Inventors: Takeda, Kazuyoshi, Kuroda, Hisao, Kaneda, Hirotaka, Hirota, Naohiko, Kaneko, Takafumi, Takoi, Kiyoshi
• Primary Examiner(s): RAGHU, GANAPATHIRAM
• Application Number:
  US10/550,528
• Publication Number:
  US 20080193593A1
• Time in Patent Office: 2,532 Days
• Field of Search: 800/320, 800/295, 426/16, 435/6, 435/189, 435/91.1, 435/25, 435/93, 536/23.1, 536/23.2
• US Class Current: 800/320
• CPC Class Codes: A01H 1/04 Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01H 6/4624 Hordeum vulgarus [barley]
• CPC Class Codes: A23L 2/38 Other non-alcoholic beverages drinks from legumes A23L11/60
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: C12C 1/00 Preparation of malt
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

A selection method for barley lipoxygenase-1 deficient barley, comprising a step of distinguishing the barley lipoxygenase-1 deficient barley by whether or not the guanine at the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene is mutated to a different base; and a method for production of malt alcoholic beverages using a material for malt alcoholic beverages derived from barley obtained by the selection method.

Claims

1. An isolated barley lipoxygenase-1 mutant gene, wherein the guanine at the splicing donor site (5′

-GT-3′

) of the 5th intron of the barley lipoxygenase-1 gene is not guanine.

2. The isolated barley lipoxygenase-1 mutant gene according to claim 1, wherein the nucleotide guanine at the splicing donor site is replaced with adenine.

7. A material for malt alcoholic beverages, wherein the material is selected from a group consisting of a seed, a malt, malt extract, barley decomposition product or processed barley derived from barley, comprising the barley lipoxygenase-1 mutant gene-according to claim 1 or 2.

9. A method for producing malt alcoholic beverages, the method comprising fermenting wort obtained from a seed, a malt, malt extract, barley decomposition product or processed barley derived from barley according to claim 7 or 8.

3. A selection method for barley lipoxygenase-1 deficient barley, the method comprising determining the presence or absence of a guanine at the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene, and selecting the barley having an adenine, thymine or cytosine at the splicing donor site.

4. The selection method for barley lipoxygenase-1 deficient barley according to claim 3, comprising selecting the barley having an adenine at the splicing donor site.

5. The selection method for barley lipoxygenase-1 deficient barley according to claim 3 or 4, wherein the determining comprises

extracting genomic DNA from a barley sample,

amplifying a DNA fragment containing at least the splicing donor site of the 5th intron of the barley lipoxygenase-1 gene from the extracted genomic DNA, and

detecting the amplified DNA fragment by cleaving with a restriction enzyme to determine the presence or absence of guanine at the splicing donor of the 5^th intron of the barley lipoxygenase-1 gene.

6. The selection method for barley lipoxygenase-1 deficient barley according to claim 5, wherein the restriction enzyme.

8. A material for malt alcoholic beverages, wherein the material is selected from a group consisting of a seed, a malt, malt extract, barley decomposition product or processed barley derived from barley selected by the selection method according to claim 3.

14. A seed, a malt, malt extract, barley decomposition product or processed barley derived from barley selected by the selection method according to claim 4.

15. A seed, a malt, malt extract, barley decomposition product or processed barley derived from barley selected by the selection method according to claim 5.

16. A seed, a malt, malt extract, barley decomposition product or processed barley derived from barley selected by the selection method according to claim 6.

17. A method for producing malt alcoholic beverages, the method comprising fermenting a seed, a malt, malt extract, barley decomposition product or processed barley derived from barley according to claim 14, 15, or 16.

10. An isolated nucleic acid comprising the nucleotide sequence from position 1 to 1554 as set forth in SEQ ID NO:

10.

11. An isolated nucleic acid comprising the nucleotide sequence as set forth in SEQ ID NO:

11.

12. An isolated nucleic acid comprising the nucleotide sequence of 10 to 60 continuous bases including the 3178th base in the nucleotide sequence as set forth in SEQ ID NO:

11.

13. A method for detecting the presence of lipoxygenase-1 (LOX-1) activity in barley, the method comprising

isolating genomic DNA from a barley sample, and

detecting the presence or absence of the 3178th base of SEQ ID NO:

11, wherein the presence of the base is an indicator of the presence of LOX-1 activity in the barley.