Diffraction grating-based encoded microparticle assay stick

• US 7,901,630 B2
• Filed: 09/13/2005
• Issued: 03/08/2011
• Est. Priority Date: 08/20/2002
• Status: Expired due to Fees

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Li, Tuo, Putnam, Martin A., Moon, John A.
• Primary Examiner(s): Siefke; Samuel P
• Assistant Examiner(s): Kilpatrick; Bryan T
• Application Number:
  US11/226,914
• Publication Number:
  US 20060072177A1
• Time in Patent Office: 2,002 Days
• Field of Search: 435/287.2, 422/173, 422/50, 422/58, 422/73, 436/164, 436/523, 436/518, 436/521
• US Class Current: 422/73
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G03H 1/26 Processes or apparatus specially adapted to produce multiple sub- holograms or to obtain images from them, e.g. multicolour technique
• CPC Class Codes: G03H 2001/0033 in hologrammetry for measuring or analysing

Abstract

An assay stick 7 includes a transparent reaction vessel or tube 14 having one or more microbeads 8 disposed therein. The microbeads 8 have a plurality of unique identification digital codes based on a diffraction grating 12 disposed therein that are detected when illuminated by incident light 24. The incident light 24 may be directed transversely onto the side or onto an end of the tube 14 with a narrow band (single wavelength) or multiple wavelength source, in which case the code is represented by a spatial distribution of light or a wavelength spectrum, respectively. The assay stick 7 may be reused or disposed upon completion of the assay. Alternatively, the beads may be attached to a strip or planar surface. The encoded beads can also provide traceability, quality-control, and authenticity of each bead 8 to its source and/or to the chemistry on each bead 8. Also, the low sample volume of the assay stick allows for faster reactions and better sensitivity.

Claims

1. An assay device comprising:

a plurality of microbeads, each microbead having at least one diffraction grating disposed therein, the grating providing an output optical signal indicative of a code when illuminated by an incident light signal; and

a reaction vessel having a cavity extending a length therein, the reaction vessel being configured to hold the microbeads within the cavity.

2. The assay device of claim 1 wherein the cavity has a cross-sectional shape and the microbeads have a common cross-sectional shape with respect to each other, the cross-sectional shape of the cavity being similar in shape to the cross-sectional shape of the microbeads.

3. The assay device of claim 1 wherein the cavity has a cross-sectional shape and the microbeads have a common cross-sectional shape with respect to each other, the cross-sectional shape of the cavity being slightly larger than the cross-sectional shape of the microbeads.

4. The assay device of claim 1 wherein the microbeads have a total length when arranged end-to-end or side-to-side within the cavity, the length of the cavity being substantially equal to the total length of the microbeads.

5. The assay device of claim 1 wherein the reaction vessel has two opposing ends with the cavity extending therebetween, the ends of the reaction vessel being configured to prevent the microbeads from falling out of the reaction vessel.

6. The assay device of claim 5 wherein at least one of the ends has an end cap coupled thereto to prevent the microbeads from falling out of the reaction vessel.

7. The assay device of claim 5 wherein at least one of the ends is at least partially collapsed into the cavity to prevent the microbeads from falling out of the reaction vessel.

8. The assay device of claim 1 wherein the microbeads are stacked end-to-end or side-to-side within the reaction vessel.

9. The assay device of claim 1 wherein the microbeads comprise a substantially single material.

10. The assay device of claim 1 wherein the microbeads have a cylindrical shape.

11. The assay device of claim 1 wherein the microbeads have a reflective coating disposed thereon.

12. The assay device of claim 1 wherein the microbeads include a magnetic or electric charge polarization.

13. The assay device of claim 1 wherein the cavity includes a plurality of cavities, each cavity extending a corresponding length within the reaction vessel.

14. The assay device of claim 1 wherein the reaction vessel has a cylindrical shape.

15. The assay device of claim 1 wherein the reaction vessel comprises a material that is transparent to an incident light signal.

16. A method of performing an assay, the method comprising:

obtaining a reaction vessel having a cavity extending a length therein, the reaction vessel including a plurality of microbeads held within the cavity, each microbead having at least one diffraction grating disposed therein;

illuminating the microbeads with an incident light signal, the grating of each microbead providing an output light signal indicative of a code; and

reading said output light signal and detecting the code therefrom.

17. The method of claim 16 further comprising conveying fluid through the cavity using a fluidic control device, the microbeads being held within the reaction vessel while the fluid is flowing therethrough.

18. The method of claim 16 wherein the cavity of the reaction vessel has a cross-sectional shape and the microbeads have a common cross-sectional shape with respect to each other, the cross-sectional shape of the cavity being similar in shape to the cross-sectional shape of the microbeads.

19. The method of claim 16 wherein the cavity of the reaction vessel has a cross-sectional shape and the microbeads have a common cross-sectional shape with respect to each other, the cross-sectional shape of the cavity being slightly larger than the cross-sectional shape of the microbeads.

20. The method of claim 16 wherein the microbeads have a total length when arranged end-to-end or side-to-side within the cavity, the length of the cavity being substantially equal to the total length of the microbeads.