Apparatus and methods for processing biological samples and a reservoir therefor
First Claim
1. An apparatus adapted for nucleic acid denaturization and hybridization of at least one biological sample on at least one carrier member in a chamber, said apparatus comprising:
- a bottom member of said chamber supporting said at least one carrier member;
a hydrophilic porous solid reservoir configured to sorb and desorb water and water vapor, said reservoir disposed inside said chamber;
a lid for the chamber configured to position the reservoir directly above the at least one biological sample such that the reservoir does not touch said at least one biological sample, when said lid is positioned to form a closed chamber;
a sample temperature controlling device within said chamber below said at least one carrier member controlling the temperature of said carrier member and said at least one biological sample, wherein said reservoir either sorbs or desorbs water and water vapor so as to control relative humidity within said closed chamber at above 85% relative humidity, when said lid is positioned to form the closed chamber; and
a reservoir temperature controlling device for controlling the temperature of said reservoir, the sample temperature controlling device and reservoir temperature controlling device being separately controlled.
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Accused Products
Abstract
An apparatus for processing at least one biological sample accommodated on at least one carrier member (15) in a chamber includes at least one reservoir (18) able to accommodate a fluid arranged on a surface inside the chamber adjacent to and/or facing a substantial part of the at least one biological sample. The apparatus may comprise a bottom member (12) arranged to support at least one carrier member (15) carrying at least one biological sample and a lid (14) including at least one fluid reservoir (18). The reservoir filled with water provides humidity to the chamber and impedes drying out of the sample.
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Citations
32 Claims
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1. An apparatus adapted for nucleic acid denaturization and hybridization of at least one biological sample on at least one carrier member in a chamber, said apparatus comprising:
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a bottom member of said chamber supporting said at least one carrier member; a hydrophilic porous solid reservoir configured to sorb and desorb water and water vapor, said reservoir disposed inside said chamber; a lid for the chamber configured to position the reservoir directly above the at least one biological sample such that the reservoir does not touch said at least one biological sample, when said lid is positioned to form a closed chamber; a sample temperature controlling device within said chamber below said at least one carrier member controlling the temperature of said carrier member and said at least one biological sample, wherein said reservoir either sorbs or desorbs water and water vapor so as to control relative humidity within said closed chamber at above 85% relative humidity, when said lid is positioned to form the closed chamber; and a reservoir temperature controlling device for controlling the temperature of said reservoir, the sample temperature controlling device and reservoir temperature controlling device being separately controlled. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. An apparatus adapted for nucleic acid denaturization and hybridization of at least one biological sample on at least one carrier member in a chamber, said apparatus comprising:
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a bottom member of said chamber supporting said at least one carrier member; a hydrophilic porous solid reservoir configured to sorb and desorb water and water vapor, said reservoir disposed inside said chamber, wherein said lid may be opened to access said at least one biological sample and said reservoir; a lid for the chamber configured to position the reservoir directly above the at least one biological sample such that the reservoir does not touch said at least one biological sample, when said lid is positioned to form a closed chamber; a sample temperature controlling device within said chamber below said at least one carrier member controlling the temperature of said carrier member and said at least one biological sample, wherein said reservoir either sorbs or desorbs water and water vapor so as to control relative humidity within said closed chamber at above 85% relative humidity, when said lid is positioned to form the closed chamber; and a second reservoir on top of said lid and outside said chamber that communicates fluid to said reservoir inside the chamber. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification