Methods of making enhanced, autologous fat grafts
First Claim
1. A method of making an enhanced, autologous fat graft comprising extracting from a subject a first portion of adipose tissue to be used as a fat graft then supplementing said fat graft with a population of concentrated, disaggregated, adipose-derived stem cells and endothelial precursor cells, wherein said stem cells and endothelial precursor cells are prepared by disaggregating and concentrating the stem cells and endothelial precursor cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.
9 Assignments
0 Petitions
Accused Products
Abstract
Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.
104 Citations
19 Claims
- 1. A method of making an enhanced, autologous fat graft comprising extracting from a subject a first portion of adipose tissue to be used as a fat graft then supplementing said fat graft with a population of concentrated, disaggregated, adipose-derived stem cells and endothelial precursor cells, wherein said stem cells and endothelial precursor cells are prepared by disaggregating and concentrating the stem cells and endothelial precursor cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.
-
10. A method of making an enhanced, autologous fat graft comprising:
-
(a) disaggregating a first portion of extracted adipose tissue from a subject with an enzyme or mechanical force to generate a cell suspension; (b) concentrating the cells in said cell suspension by buoyant density sedimentation, filtration or centrifugation to obtain a population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells; and (c) supplementing a second portion of extracted adipose tissue to be used as a fat graft from said subject with the population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells, so as to make said enhanced, autologous fat graft. - View Dependent Claims (11, 12, 13, 14, 15)
-
- 16. A method of making an enhanced, autologous fat graft comprising extracting from a subject a first portion of adipose tissue to be used as a fat graft then supplementing said fat graft with a population of concentrated, disaggregated adipose-derived stem cells, wherein said stem cells are prepared by disaggregating and concentrating the stem cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.
Specification