Markers for prenatal diagnosis and monitoring

• US 7,901,884 B2
• Filed: 04/06/2007
• Issued: 03/08/2011
• Est. Priority Date: 05/03/2006
• Status: Active Grant

• Current Assignee: Chinese University of Hong Kong
• Original Assignee: Chinese University of Hong Kong
• Inventors: Lo, Yuk Ming Dennis, Ding, Chunming, Jin, Shengnan, Chim, Stephen Siu Chung, Chiu, Rossa Wai Kwun, Lun, Fiona Miu Fun, Lee, Tracy Yuen Han
• Primary Examiner(s): GOLDBERG, JEANINE ANNE
• Application Number:
  US11/784,499
• Publication Number:
  US 20070275402A1
• Time in Patent Office: 1,432 Days
• Field of Search: None
• US Class Current: 435/6.11
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2523/125 Bisulfite(s)
• CPC Class Codes: C12Q 2600/154 Methylation markers
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

This application provides the use of novel fetal markers for prenatal diagnosis and monitoring of certain pregnancy-related conditions. More specifically, the invention resides in the discovery that certain CpG islands located on fetal chromosome 21 demonstrate a methylation profile that is distinct from that of the corresponding CpG islands located on maternal chromosome 21. This application also provides kits for diagnosing or monitoring of the relevant conditions.

Claims

1. A method for detecting trisomy 21 in a fetus carried by a pregnant woman, comprising the steps of:

(a) obtaining a biological sample from the woman, wherein the sample is whole blood, serum, or plasma;

(b) determining the methylation status of a CpG-containing genomic sequence in the sample, wherein the genomic sequence from the fetus and the genomic sequence from the woman are differentially methylated, thereby distinguishing the genomic sequence from the woman and the genomic sequence from the fetus in the sample, wherein the genomic sequence is at least 15 nucleotides in length, comprising at least one cytosine, and is within genomic locus Holocarboxylase Synthetase (HLCS) on chromosome 21;

(c) determining the amount of the methylated genomic sequence from the fetus; and

(d) comparing the amount of the methylated genomic sequence from the fetus with a standard control, wherein an increase from the standard control indicates the presence of trisomy 21.

2. The method of claim 1, wherein the genomic sequence from the woman is unmethylated and the genomic sequence from the fetus is methylated.

3. The method of claim 1, wherein step (b) is performed by treating the sample with a reagent that differentially modifies methylated and unmethylated DNA.

4. The method of claim 3, wherein the reagent comprises bisulfite.

5. The method of claim 3, wherein the reagent comprises one or more enzymes that preferentially cleave methylated DNA.

6. The method of claim 3, wherein the reagent comprises one or more enzymes that preferentially cleave unmethylated DNA.

7. The method of claim 1, wherein step (b) is performed by methylation-specific PCR.

27. The method of claim 1, 8, or 14, wherein the genomic sequence is a CpG island.

8. A method for detecting trisomy 21 in a fetus carried by a pregnant woman, comprising the steps of:

(a) obtaining DNA in a biological sample from the woman, wherein the sample is whole blood, serum, or plasma;

(b) treating the DNA from step (a) with bisulfite; and

(c) performing an amplification reaction using the DNA from step (b) and two primers to amplify a CpG-containing genomic sequence, wherein the genomic sequence is at least 15 nucleotides in length, comprises at least one cytosine, and is within genomic locus Holocarboxylase Synthetase (HLCS) on chromosome 21;

and wherein at least one of the two primers binds differentially to the genomic sequence from the fetus; and

(d) comparing the amount of the amplified portion of the genomic sequence from step (c) with a standard control, wherein an increase from the standard control indicates the presence of trisomy 21.

9. The method of claim 8, wherein the amplification reaction is a polymerase chain reaction (PCR).

10. The method of claim 8, wherein the amplification reaction is a methylation-specific PCR.

11. The method of claim 8, wherein the amplification reaction is a nucleic acid sequence based amplification.

12. The method of claim 8, wherein the amplification reaction is a strand displacement reaction.

13. The method of claim 8, wherein the amplification reaction is a branched DNA amplification reaction.

14. A method for detecting trisomy 21 in a fetus carried by a pregnant woman, comprising the steps of:

(a) obtaining a biological sample from the woman, wherein the sample is whole blood, serum, or plasma;

(b) determining the amount of a CpG-containing genomic sequence in the sample, wherein the genomic sequence is at least 15 nucleotides in length, comprises at least one methylated cytosine, and is within genomic locus Holocarboxylase Synthetase (HLCS) on chromosome 21; and

(c) comparing the amount of the genomic sequence with a standard control, wherein an increase from the standard control indicates the presence of trisomy 21.

15. The method of claim 14, wherein step (b) comprises treating DNA present in the blood sample with a reagent that differentially modifies methylated and unmethylated cytosine.

16. The method of claim 15, wherein the reagent comprises bisulfite.

17. The method of claim 15, wherein the reagent comprises one or more enzymes that preferentially cleave DNA comprising methylated cytosine.

18. The method of claim 15, wherein the reagent comprises one or more enzymes that preferentially cleave DNA comprising unmethylated cytosine.

19. The method of claim 14, wherein step (b) comprises an amplification reaction.

20. The method of claim 19, wherein the amplification reaction is a polymerase chain reaction (PCR).

21. The method of claim 20, wherein the PCR is a methylation-specific PCR.

22. The method of claim 19, wherein the amplification reaction is a nucleic acid sequence based amplification.

23. The method of claim 19, wherein the amplification reaction is a strand displacement reaction.

24. The method of claim 19, wherein the amplification reaction is a branched DNA amplification reaction.

25. The method of claim 14, wherein the amount of the genomic DNA sequence is determined by electrophoresis.

26. The method of claim 14, wherein the amount of the genomic DNA sequence is determined by polynucleotide hybridization.