High voltage nanosecond pulse generator using fast recovery diodes for cell electro-manipulation

• US 7,901,930 B2
• Filed: 02/09/2010
• Issued: 03/08/2011
• Est. Priority Date: 04/22/2005
• Status: Active Grant

• Current Assignee: University of Southern California
• Original Assignee: University of Southern California
• Inventors: Gundersen, Martin A., Kuthi, Andras
• Primary Examiner(s): Beisner; William H
• Assistant Examiner(s): HOBBS, MICHAEL L
• Application Number:
  US12/703,002
• Publication Number:
  US 20100141043A1
• Time in Patent Office: 392 Days
• Field of Search: 435/173.6, 435/283.1, 435/285.2, 435/461, 327/304
• US Class Current: 435/285.2
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: H03K 17/74 by the use, as active elements, of diodes by the use of more than one type of semiconductor device H03K17/567; by the use of tunnel diodes H03K17/58; by the use of negative resistance diodes H03K17/70
• CPC Class Codes: H03K 3/57 the switching device being a semiconductor device
• CPC Class Codes: H03K 5/12 by steepening leading or trailing edges

Abstract

A pulse generator circuit may include a diode configured to operate as an opening switch, a tank circuit in series with the diode having an admittance that is switchable from a first value to a second value that is different from the first value, and a switching system configured to cause the tank circuit to switch between the first value and the second value. The diode may saturate in less than 100 nanoseconds. A saturable core transformer may operate as a switch that controls the opening of the diode. The pulse generator may generate a plurality of pulses, each having a length of no more than 3 nanoseconds and an amplitude of at least 1 kilovolt. Electrodes may be connected to the pulse generator to deliver the plurality of pulses to biological cells.

Claims

1. A pulse generator circuit comprising:

a diode that that is configured to operate as an opening switch and that saturates in less than 100 nanoseconds; and

a saturable core transformer configured to operate as a switch that controls the opening of the diode.

2. The pulse generator of claim 1 further comprising a tank circuit that includes the saturable core transformer and that is in series with the diode.

3. The pulse generator of claim 2 wherein the tank circuit has an admittance that is switchable from a first value to a second value that is different from the first value.

4. The pulse generator of claim 3 wherein the saturable core transformer is configured to cause the admittance to switch from the first value to the second value.

5. The pulse generator of claim 3 wherein the first value of the admittance is approximately half of the second value.

6. The pulse generator of claim 2 wherein the tank circuit include a first and a second capacitance.

7. The pulse generator of claim 6 wherein the diode is configured to be forward biased and reversed biased at different times and further comprising a MOSFET configured to cause the first and the second capacitance to be part of the tank circuit while the diode is forward biased and to cause the second capacitance to be part of the tank circuit, but not the first capacitance, while the diode is reversed biased.