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Efficient arrays of amplified polynucleotides

  • US 7,910,302 B2
  • Filed: 10/29/2007
  • Issued: 03/22/2011
  • Est. Priority Date: 10/27/2006
  • Status: Active Grant
First Claim
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1. A method of making a random array of amplified polynucleotides, said method comprising the steps of:

  • a) providing;

    i) a surface, which comprises capture probes, wherein said capture probes have free 3′

    ends, andii) a plurality of concatemers disposed on said surface, wherein each concatemer is bound to capture probes at a specific position on said surface and wherein each concatemer comprises multiple copies of a monomer, and said monomer comprises a first interspersed adaptor that is located between a first and second target sequence of a target polynucleotide, wherein said first and second target sequences are contiguous segments of said target polynucleotide; and

    b) extending said capture probes such that said concatemers disposed on said surface are amplified;

    wherein the amplification products of said concatemers are attached to the surface at or near the specific position of the concatemer amplified, thereby making said random array of amplified polynucleotides.

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