Method and device for measuring dynamic parameters of particles

• US 7,912,274 B2
• Filed: 01/17/2006
• Issued: 03/22/2011
• Est. Priority Date: 01/17/2005
• Status: Active Grant

• Current Assignee: Biophos AG
• Original Assignee: Biophos AG
• Inventors: Rigler, Per, Nilsson, Lennart, Rigler, Rudolf
• Primary Examiner(s): Chawan; Sheela C
• Application Number:
  US11/814,141
• Publication Number:
  US 20080195334A1
• Time in Patent Office: 1,890 Days
• Field of Search: 382/100, 382/141, 356/28, 356/27, 356/335, 356/336, 356/28.5, 702/29, 702/179, 738/610.6, 708/250, 386/E5.043, 386/270, 331/78
• US Class Current: 382/141
• CPC Class Codes: G01N 15/06 Investigating concentration of particle suspensions by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10
• CPC Class Codes: G01N 15/075 by optical means
• CPC Class Codes: G01N 2015/0003 Determining electric mobility, velocity profile, average speed or velocity of a plurality of particles

Abstract

The present invention relates to a method and device for measuring dynamic parameters of particles comprises applying time correlation analysis on fluctuation of the particles with respect to a detection area of a digital picture.

Claims

1. A method for measuring dynamic parameters of particles, characterised in that the method comprises applying correlation analysis on temporal fluctuations of the number of particles with respect to a detection area of a digital picture, wherein a time correlation function or functions is/are calculated based on the temporal fluctuations of the number of particles.

2. The method according to claim 1, characterised in that the digital picture is generated by an image sensor.

3. The method according to claim 1, wherein the method further comprises background subtraction.

4. The method according to claim 1, wherein the particles are spermatozoa.

5. The method according to claim 1, wherein the method further comprises a measurement of the particle concentration.

6. A method for measuring dynamic parameters of particles, characterised in that the method comprises detecting the particles by a detector capable of generating a digital picture, and applying correlation analysis based on temporal fluctuations of the number of particles with respect to a detection area of the digital picture, wherein a time correlation function or functions is/are calculated based on the temporal fluctuations of the number of particles.

7. A method for measuring dynamic parameters of particles, characterised in that the method comprises providing the particles in a solution, a light source, an image sensor, computational means, detecting the events on a digital picture generated by the image sensor, and calculating the correlation function based on temporal fluctuations of the number of particles with respect to a detection area of the digital picture, wherein a time correlation function or functions is/are calculated based on the temporal fluctuations of the number of particles.

8. The method according to claim 7, characterised in that optical means are positioned in the light path between the object plane and the plane of the image sensor.

9. The method according to claim 7, characterised in that the correlation function is based on fluctuations of particles with respect to a plurality of detection areas.

10. The method according to claim 7, characterised in that the image sensor is of solid state detector type.

11. A device for measuring dynamic parameters of particles in a solution, characterised in that the device comprises a sample compartment, a light source, an image sensor, computational means for processing signals from the image sensor, detecting the particles on a digital picture generated by the image sensor, and calculating the correlation function based on temporal fluctuations of the number of particles with respect to a detection area of the digital picture, wherein a time correlation function or functions is/are calculated based on the temporal fluctuations of the number of particles.

12. The device according to claim 11, characterised in that the image sensor is of solid state type.

13. The device according to claim 11, characterised in that optical means are positioned in the light path between the object plane and the plane of the image sensor.

14. The device according to claims 11, characterised in that the correlation function or functions is based on fluctuations of particles with respect to a plurality of detection areas.

15. The device according to claims 11, characterised in that the particles are spermatozoa.

16. The device according to claim 11, characterised in that it comprises means for measuring the particle concentration.

17. Use of a device as defined by claim 11 for measuring dynamic parameters.

18. The use of claim 17 for further measuring the particle concentration.