Apparatus and method for separating and concentrating fluids containing multiple components
First Claim
Patent Images
1. A method of enriching a scaffold for application relative to an anatomy, comprising:
- connecting a first piston and a second piston with a connection member;
obtaining a volume of a first whole material;
placing at least a portion of the obtained volume of the first whole material into a container;
forming a first fraction of the first whole material by centrifuging the placed portion of the obtained volume of the first whole material placed in the container;
disposing the first piston relative to the second piston;
obtaining a volume of a second whole material that is different from the first whole material;
placing at least a portion of the obtained volume of the second whole material into the container;
forming a second fraction of the second whole material by centrifuging the placed portion of the obtained volume of the second whole material placed in the container;
containing at least a portion of both the first fraction and the second fraction near a first collection surface of the first piston; and
applying a selected portion of the first fraction and the second fraction to the scaffold.
8 Assignments
0 Petitions
Accused Products
Abstract
An apparatus is disclosed that allows for separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at least three fractions in the apparatus. It also provides for a new method of extracting the buffy coat phase from a whole blood sample. A buoy system that may include a first buoy portion and a second buoy member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient.
-
Citations
17 Claims
-
1. A method of enriching a scaffold for application relative to an anatomy, comprising:
-
connecting a first piston and a second piston with a connection member; obtaining a volume of a first whole material; placing at least a portion of the obtained volume of the first whole material into a container; forming a first fraction of the first whole material by centrifuging the placed portion of the obtained volume of the first whole material placed in the container; disposing the first piston relative to the second piston; obtaining a volume of a second whole material that is different from the first whole material; placing at least a portion of the obtained volume of the second whole material into the container; forming a second fraction of the second whole material by centrifuging the placed portion of the obtained volume of the second whole material placed in the container; containing at least a portion of both the first fraction and the second fraction near a first collection surface of the first piston; and applying a selected portion of the first fraction and the second fraction to the scaffold. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
-
10. A system for enriched scaffolding for application relative to an anatomy, comprising:
-
a piston system including a first piston and a second piston connected with a connection member; a container operable to contain a volume of a first whole material and the piston system; and a collection area defined by the first piston; wherein a first fraction of a first whole material is operable to be formed in the container by centrifuging the first whole material contained in the container; wherein at least a portion of the first fraction is operable to be contained in the collection area of the first piston; and wherein the first piston is operable to be moved relative to the second piston as constrained by the connection member; wherein the second piston includes a second surface substantially complementary and mateable to the upper surface of the first piston; wherein the first fraction is operable to be applied to a scaffold. - View Dependent Claims (11)
-
-
12. A method of enriching a scaffold for application relative to an anatomy, comprising:
-
connecting a first piston and a second piston with a connection member; obtaining a volume of a bone marrow aspirate material; placing at least a portion of the obtained volume of the bone marrow aspirate material into a container; forming a first fraction of the bone marrow aspirate material by centrifuging the placed portion of the obtained volume of the bone marrow aspirate material placed in the container; containing at least a portion of the first fraction in a collection area of the first piston; disposing the first piston relative to the second piston; and applying the first fraction to the scaffold. - View Dependent Claims (13, 14, 15, 16, 17)
-
Specification