Tissue matrices comprising placental stem cells, and methods of making the same
First Claim
1. A method of preparing a tissue matrix, comprising seeding human placental stem cells into or onto a decellularized or synthetic tissue matrix, wherein said stem cells are from a human placenta that has been drained of cord blood and perfused to remove residual blood, and comprise stem cells that are CD34−
- or OCT-4+, wherein said placental stem cells are SSEA4−
.
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Accused Products
Abstract
A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.
264 Citations
55 Claims
-
1. A method of preparing a tissue matrix, comprising seeding human placental stem cells into or onto a decellularized or synthetic tissue matrix, wherein said stem cells are from a human placenta that has been drained of cord blood and perfused to remove residual blood, and comprise stem cells that are CD34−
- or OCT-4+, wherein said placental stem cells are SSEA4−
. - View Dependent Claims (2, 3, 4, 5, 6, 7, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 54)
- or OCT-4+, wherein said placental stem cells are SSEA4−
-
8. A decellularized or synthetic tissue matrix comprising human placental stem cells that are CD34−
- or OCT-4+, wherein said placental stem cells are SSEA4−
. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 32, 33, 34, 35)
- or OCT-4+, wherein said placental stem cells are SSEA4−
-
36. A tissue comprising a decellularized organ and a plurality of CD34−
- or OCT-4+ human placental stem cells, wherein said placental stem cells are SSEA4−
. - View Dependent Claims (37, 38, 39)
- or OCT-4+ human placental stem cells, wherein said placental stem cells are SSEA4−
-
40. A method of producing a tissue comprising contacting a decellularized organ with a plurality of CD34−
- or OCT-4+ human placental stem cells, wherein said placental stem cells are SSEA4−
.
- or OCT-4+ human placental stem cells, wherein said placental stem cells are SSEA4−
-
41. A method of transplanting a tissue into a recipient, comprising transplanting said tissue into a recipient, wherein said tissue comprises a decellularized organ and a plurality of CD34−
- or OCT-4+ human placental stem cells, wherein said placental stem cells are SSEA4−
, and wherein said placental stem cells are from a human placenta that has been drained of cord blood and perfused to remove residual blood. - View Dependent Claims (42, 43)
- or OCT-4+ human placental stem cells, wherein said placental stem cells are SSEA4−
-
44. A tissue matrix comprising isolated CD34−
- , CD10+, SH2+ multipotent placental cells.
- View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53)
-
55. A tissue matrix comprising isolated human multipotent placental cells, wherein said placental stem cells are CD29+, CD90+, SH2+, SH3+, SH4+, CD34−
- , and CD45−
.
- , and CD45−
Specification