Process for starch liquefaction and fermentation
First Claim
1. A method of reducing the viscosity of a mash comprising the steps of,a) milling plant material comprising between about 0.5% to about 100% admix of a transgenic plant expressing at least one starch-digesting enzyme;
- b) combining milled plant material with a liquid to form a mash in a commercial slurry tank; and
c) hydrolyzing the starch in the mash to reach a viscosity of less than 3000 cP.
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Abstract
The presently disclosed subject matter provides improved processes for processing starch from plant sources, including processes for starch liquefaction, for simultaneous liquefaction and saccharification, and for the preparation of ethanol. These processes can be performed without a pH adjustment and at relatively low temperatures. The processes can involve the use of starch-containing plant material derived from plants that express starch-digesting enzymes. The presently disclosed subject matter further relates to improved processes for the preparation of other starch-derived products, including dried distiller grain (dried distiller grain) and dried distiller grain and solubles (dried distiller grain and solubles), and to the starch-derived products, themselves.
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Citations
33 Claims
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1. A method of reducing the viscosity of a mash comprising the steps of,
a) milling plant material comprising between about 0.5% to about 100% admix of a transgenic plant expressing at least one starch-digesting enzyme; -
b) combining milled plant material with a liquid to form a mash in a commercial slurry tank; and c) hydrolyzing the starch in the mash to reach a viscosity of less than 3000 cP. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method of reducing dough ball formation comprising the steps of,
a) milling plant material comprising between about 0.5% to about 100% admix of a transgenic plant material expressing at least one starch-digesting enzyme; b) combining milled plant material with a liquid to form a mash in a slurry tank wherein the milled plant material does not form dough balls that can be detected in the effluent from the slurry tank. - View Dependent Claims (13, 14, 15, 16, 17)
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18. A method of reducing the viscosity of a mash comprising the steps of,
a) milling plant material comprising between about 0.5% to about 100% admix of a transgenic plant material expressing at least one starch-digesting enzyme; -
b) combining milled plant material with a liquid to form a mash in a commercial slurry tank; c) hydrolyzing the starch in the mash; d) cooling the slurry to about ambient temperature wherein the viscosity of the cooled mash is less than about 2000 cP at about ambient temperature. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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29. A method of increasing total solids of a mash while maintaining a desired viscosity comprising the steps of:
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a) milling plant material comprising between about 0.5% to about 100% admix of a transgenic plant expressing at least one starch-digesting enzyme; b) combining milled plant material with a liquid to form a mash in a slurry tank, wherein the total solids of the mash consists of about 35% to about 60% milled plant material; and c) hydrolyzing the starch in the mash to reach a desired viscosity. - View Dependent Claims (30, 31, 32, 33)
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Specification