Method for separating an analyte from a sample
First Claim
1. A method for extracting nucleic acid from a sample, the sample containing cells, spores, or microorganisms, the method comprising:
- a) introducing the sample into a cartridge having;
i) a lysing region for lysing the cells, spores, or microorganisms to release the nucleic acid therefrom; and
ii) a capture region, the capture region comprising a channel or chamber containing capture material for capturing the nucleic acid;
b) drying the sample on paper or membrane material in the lysing region, the paper or membrane material being impregnated with at least one chemical for lysing the cells, spores, or microorganisms in the sample to release the nucleic acid from the cells, spores, or microorganisms, while binding contaminants or inhibitors in the sample to the paper or membrane material;
c) placing fluid in the lysing region and releasing the nucleic acid from the paper or membrane material into the fluid while the contaminants or inhibitors remain bound to the paper or membrane material;
d) forcing the fluid containing the nucleic acid to flow through the capture region to capture the nucleic acid with the capture material; and
e) eluting the nucleic acid from the capture region.
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Accused Products
Abstract
An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
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Citations
25 Claims
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1. A method for extracting nucleic acid from a sample, the sample containing cells, spores, or microorganisms, the method comprising:
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a) introducing the sample into a cartridge having; i) a lysing region for lysing the cells, spores, or microorganisms to release the nucleic acid therefrom; and ii) a capture region, the capture region comprising a channel or chamber containing capture material for capturing the nucleic acid; b) drying the sample on paper or membrane material in the lysing region, the paper or membrane material being impregnated with at least one chemical for lysing the cells, spores, or microorganisms in the sample to release the nucleic acid from the cells, spores, or microorganisms, while binding contaminants or inhibitors in the sample to the paper or membrane material; c) placing fluid in the lysing region and releasing the nucleic acid from the paper or membrane material into the fluid while the contaminants or inhibitors remain bound to the paper or membrane material; d) forcing the fluid containing the nucleic acid to flow through the capture region to capture the nucleic acid with the capture material; and e) eluting the nucleic acid from the capture region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification