Methods for detecting sleepiness

• US 7,915,005 B2
• Filed: 11/09/2006
• Issued: 03/29/2011
• Est. Priority Date: 11/09/2005
• Status: Active Grant

• Current Assignee: Washington University In St Louis
• Original Assignee: Washington University In St Louis
• Inventors: Seugnet, Laurent, Shaw, Paul, Boero, Jaime, Duntley, Stephen
• Primary Examiner(s): Hindenburg; Max
• Assistant Examiner(s): DANEGA, RENEE A
• Application Number:
  US11/558,074
• Publication Number:
  US 20070105180A1
• Time in Patent Office: 1,601 Days
• Field of Search: 435/22, 600/573
• US Class Current: 435/22
• CPC Class Codes: C12Q 1/40 involving amylase
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G01N 2800/2864 Sleep disorders

Abstract

The invention generally provides a biomarker for sleepiness, a method for detecting sleepiness, and a method of identifying nucleic acids associated with sleepiness.

Claims

1. A method for identifying a subject that is awake and sleep deprived, the method comprising:

(a) collecting a biosample from the subject,

(b) processing the biosample to measure the level of amylase,

(c) measuring the level of amylase in the biosample,

(d) comparing the level of amylase in the biosample to a baseline level of amylase, and

(e) identifying the awake subject as sleepy and sleep deprived if the biosample has an elevated level of amylase compared to a baseline level of amylase.

2. The method of claim 1, wherein the biosample is selected from the group consisting a blood sample, an interstitial fluid sample, a saliva sample, a urine sample, and a pancreatic sample.

3. The method of claim 1, wherein the biosample is a saliva sample.

4. The method of claim 1, wherein the level of amylase measured is selected from the group consisting of the level of amylase mRNA, the level of amylase transcription, the level of amylase translation, the level of amylase protein, and the level of amylase activity.

5. The method of claim 1, wherein the level of amylase mRNA is measured.

6. The method of claim 1, wherein the subject has been diagnosed as having a sleep disorder.

7. The method of claim 1, wherein the subject is at risk for sleepiness.

8. The method of claim 7, wherein sleepiness is a condition selected from the group consisting of coma, dyssomnias, parasomnias, and sleep disorders.

9. The method of claim 5, wherein the level of mRNA is measured by a technique selected from the group consisting of northern blot, microarray, expression profiling, nuclease protection assay, RNase protection assay, S1 nuclease protection assay, RT-PCR, quantitative RT-PCR, in situ hybridization, or variants thereof.

11. The method of claim 1, wherein the biosample is selected from the group consisting a blood sample, an interstitial fluid sample, a saliva sample, a urine sample, and a pancreatic sample.

12. The method of claim 1, wherein the biosample is a saliva sample.

13. The method of claim 1, wherein the level of amylase measured is selected from the group consisting of the level of amylase mRNA, the level of amylase transcription, the level of amylase translation, the level of amylase protein, and the level of amylase activity.

14. The method of claim 1, wherein the level of amylase mRNA is measured.

15. The method of claim 14, wherein the level of mRNA is measured by a technique selected from the group consisting of northern blot, microarray, expression profiling, nuclease protection assay, RNase protection assay, S1 nuclease protection assay, RT-PCR, quantitative RT-PCR, in situ hybridization, or variants thereof.

10. A method for diagnosing a sleep disorder in a subject, the method comprising:

(a) collecting a biosample from the subject,

(b) processing the biosample to measure the level of amylase,

(c) measuring the level of amylase in the biosample,

(d) comparing the level of amylase in the biosample to a baseline level of amylase, and

(e) diagnosing a sleep disorder if the biosample has an elevated level of amylase compared to a baseline level of amylase.