Methodologies, processes and automated devices for the orientation, sampling and collection of seed tissues from individual seed
First Claim
1. A method of sampling seed comprising:
- applying a magnetic substance to a particular location on a seed corresponding to a desired pre-determined orientation of the seed;
automatically positioning the seed in the pre-determined orientation by influence of a magnetic field;
removing a measurable sample from the seed.
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Accused Products
Abstract
Apparatus, methods, and systems for high throughput, useful sampling of seed, wherein viability is optionally maintained, are disclosed. Seed from one generation in a plant advancement experiment is individually sampled by removal and collection of tissue from the seed. The tissue is then processed to derive one or more biochemical, genetic, or phenotypic characteristic of the seed before a decision is made whether to utilize that seed further in a plant advancement experiment or other plant research and development. In some embodiments of the method, the sampling is controlled to remove a useful amount of tissue for analytical purposes without significant effect on viability potential of the sampled seed. In some embodiments, the sampling is controlled to deter contamination of the sample. In some embodiments, the seed is automatically positioned and oriented to facilitate efficient and accurate sampling.
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Citations
17 Claims
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1. A method of sampling seed comprising:
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applying a magnetic substance to a particular location on a seed corresponding to a desired pre-determined orientation of the seed; automatically positioning the seed in the pre-determined orientation by influence of a magnetic field; removing a measurable sample from the seed. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of sampling seed comprising:
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applying a magnetically active substance to a particular location on a seed corresponding to a desired pre-determined orientation of the seed; magnetically attracting the magnetically active substance on the seed to automatically position the seed in the desired pre-determined orientation; and removing a measurable sample from the seed while being retained in the desired pre-determined orientation. - View Dependent Claims (8, 9, 10, 11, 12, 13)
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14. A method of sampling seed comprising:
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identifying a single location on a seed corresponding to a pre-determined orientation of the seed for facilitating sampling; applying a magnetically active substance to the single location on the seed before sampling; subjecting the seed to influence of a magnetic field at a position corresponding with the step of sampling; attracting the magnetically active substance to automatically position and orient the seed in the pre-determined orientation; and removing a measurable sample from the seed. - View Dependent Claims (15, 16, 17)
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Specification