Method for producing a recipient-specific tissue transplant or tissue implant

• US 7,915,038 B2
• Filed: 05/28/2001
• Issued: 03/29/2011
• Est. Priority Date: 05/29/2000
• Status: Expired due to Fees

• Current Assignee: Augustinus Bader
• Original Assignee: Augustinus Bader
• Inventors: Bader, Augustinus
• Primary Examiner(s): DRISCOLL, LORA E BARNHART
• Application Number:
  US10/296,672
• Publication Number:
  US 20040028662A1
• Time in Patent Office: 3,592 Days
• Field of Search: 435/325, 435/347, 435/366, 435/373, 435/384, 435/395, 435/1.1
• US Class Current: 435/325
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/383 Nerve cells, e.g. dendritic cells, Schwann cells
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: C12N 5/0062 General methods for three-dimensional culture

Abstract

The method represents a further development of a cell-colonization process, in which biological cells are colonized on a synthetic or natural tissue matrix in order to obtain a tissue implant or tissue transplant. The growth of the cells, which in general come from the designated recipient of the transplant of implant, may be promoted by the addition of mediators, factors or co-factors. The factors or mediators are supplied by co-cultivating cells, which are particularly suitable for producing the factors, with the tissue.

Claims

1. A method for preparing a recipient-specific tissue transplant or tissue implant, comprising the steps of:

(a) providing a synthetic matrix onto which cells have been applied that are autologous to the recipient of the transplant or implant and are selected from the group consisting of connective tissue cells, muscle cells, endothelial cells, skin cells, and organ cells selected from heart and kidney cells; and

(b) cultivating said synthetic matrix of step (a) in a first culture solution that is separated by means of a film, membrane or partition from a second culture, said second culture comprising macrophage that are autologous to the recipient, thereby preparing the recipient-specific implant or transplant.

2. The method as claimed in claim 1, wherein said recipient-specific tissue transplant or tissue implant is a heart valve transplant or a heart valve implant.