Cellular microarray and its microfabrication method

• US 7,919,048 B2
• Filed: 04/25/2008
• Issued: 04/05/2011
• Est. Priority Date: 10/05/2007
• Status: Expired due to Fees

• Current Assignee: National Taiwan University (Government of The Republic of China)
• Original Assignee: National Taiwan University (Government of The Republic of China)
• Inventors: Yang, Chun-Hui, Lee, Hsin-Yu, Hsiung, Lo-Chang, Wo, Andrew M.
• Primary Examiner(s): Bullock; In Suk
• Assistant Examiner(s): Kingan; Timothy G
• Application Number:
  US12/149,030
• Publication Number:
  US 20090093376A1
• Time in Patent Office: 1,075 Days
• Field of Search: 506/14, 204/547, 422/101, 435/174, 435/283.1, 436/177
• US Class Current: 422/504
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A cellular microarray is disclosed, which has a substrate, multiple first conductive lines, multiple second conductive lines, and multiple PIREs arranged on the surface of the substrate in an array. Each PIRE includes multiple first ring-shaped electrodes, and multiple second ring-shaped electrodes. The first ring-shaped electrodes, and the second ring-shaped electrodes are located on the surface of the substrate alternately in each PIRE. Moreover, the outermost ring-shaped electrodes of any two adjacent feather-shaped electrodes are different. The disclosed cellular microarray can adhere the cells rapidly and uniformly, increase the output of manufacturing, and reduce the cost for manufacturing and application.

Claims

1. A cellular microarray, comprising:

I) a substrate;

2) a plurality of first conductive lines located on a surface of the substrate;

3) a plurality of second conductive lines located on the surface of the substrate; and

4) a plurality of paired planar interdigitated electrodes arranged on the surface of the substrate in an array, wherein;

each paired planar interdigitated electrode comprises two members, a first electrode and a second electrode;

the first and second conductive lines are electrically connected to the opposite poles of a voltage source;

the first conductive lines are electrically connected to the first electrodes of each paired planar interdigitated electrode;

the second conductive lines are electrically connected to the second electrodes of each paired planar interdigitated electrode;

one member of each paired planar interdigitated electrode is interdigitated with the other member of the pair;

one member of each paired planar interdigitated electrode comprises a shaped portion that partially surrounds the other member of the pair;

the first electrodes and the second electrodes of each paired planar interdigitated electrode are located on the surface of the substrate; and

each of the first and second electrodes of adjacent paired planar interdigitated electrodes partially surrounds and is partially surrounded by the second and first electrodes, respectively, on the surface of the substrate.

2. The cellular microarray of claim 1, wherein the shaped portion of each paired planar interdigitated electrode forms an open polygon, and parts of the first electrodes are linear electrodes or open poly-segmental electrodes.

3. The cellular microarray of claim 1, wherein the shaped portion of each paired planar interdigitated electrode form an open circle or an open ellipse, and parts of the first electrode are arc-shaped electrodes.

4. The cellular microarray of claim 3, wherein the first electrode and the second electrode are arranged in a form of concentric open circles.

5. The cellular microarray of claim 1, wherein the gaps between the first electrode and the second electrode are the same in each paired planar interdigitated electrode.

6. The cellular microarray of claim 1, wherein the widths of the first electrode and widths of the second electrode are the same in each paired planar interdigitated electrode.

7. The cellular microarray of claim 1, wherein the first conductive lines and the second conductive lines are arranged alternately on the surface of the substrate, and each paired planar interdigitated electrode is set between a first conductive line and a second conductive line.

8. The cellular microarray of claim 1, wherein the first conductive lines and the second conductive lines are parallel with each other.

9. The cellular microarray of claim 1, wherein the paired planar interdigitated electrodes form an m×

n array, and each m and n is independently an integer of 1 or more.

10. The cellular microarray of claim 1, wherein the shaped portion of the first electrode of each paired planar interdigitated electrode comprises a plurality of thorns.

11. The cellular microarray of claim 1, wherein the shaped portion of the second electrode of each paired planar interdigitated electrode comprises a plurality of thorns.

12. The cellular microarray of claim 10, wherein every distance between the adjacent thorns is the same.

13. The cellular microarray of claim 11, wherein every distance between the adjacent thorns is the same.

14. The cellular microarray of claim 1 wherein the first electrode or the second electrode are metal electrodes or transparent electrodes.

15. The cellular microarray of claim 14, wherein the transparent electrodes are ITO electrodes or IZO electrodes.

16. The cellular microarray of claim 1, further comprising a cell adhesion layer located on a surface of the paired planar interdigitated electrodes or on the surface of the substrate.

17. The cellular microarray of claim 1, further comprising a concentration gradient generator comprising a substrate-forming enclosed channel and/or chambers located above the surface of the substrate to protect the substrate and the plurality of paired planar interdigitated electrodes.

18. The cellular microarray of claim 1, further comprising a concentration gradient generating channel, and a plurality of flow paths, wherein the concentration gradient generating channel and the flow paths are located on the surface of the substrate, and the flow paths connect the concentration gradient generating channel and the paired planar interdigitated electrodes.