Quantitative dual-dye photometric method for determining dilution impact
First Claim
1. A method of measuring a liquid volume of a sample solution in a vessel, wherein the vessel includes a bottom and known dimensions, the method comprising the steps of:
- a. adding to the vessel a diluent including a known concentration of a diluent chromophore which absorbs light at a second wavelength;
b. measuring the absorbance of the diluent chromophore at the second wavelength;
c. adding a volume of the sample solution to the vessel, wherein the sample solution includes a known concentration of a sample solution chromophore which absorbs light at a first wavelength, and wherein the sample solution does not include the diluent chromophore;
d. mixing the diluent and the sample solution in the vessel to produce a mixture of the sample solution and the diluent;
e. measuring the absorbance of the mixture of the sample solution and the diluent at the first wavelength and at the second wavelength; and
f. calculating the volume of the sample solution added to the vessel based on the measured absorbances at the first wavelength and the second wavelength.
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Abstract
The invention provides ways to determine the impact of diluting a solution wherein the diluting may be carried out for any of a variety of purposes. In one embodiment, the method enables accurate volume dispensation calculations independent of meniscus shape. In another embodiment, the method enables accurate determination of plate washing efficiency. In yet another embodiment, the method enables the accurate determination of dilution ratio over a plurality of dilution steps. The methods described may be carried out using one or more systems arranged to perform the steps. A kit of the invention includes instructions for carrying out the steps of the methods and, optionally, one or more solutions suitable for conducting photometric measurements.
42 Citations
7 Claims
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1. A method of measuring a liquid volume of a sample solution in a vessel, wherein the vessel includes a bottom and known dimensions, the method comprising the steps of:
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a. adding to the vessel a diluent including a known concentration of a diluent chromophore which absorbs light at a second wavelength; b. measuring the absorbance of the diluent chromophore at the second wavelength; c. adding a volume of the sample solution to the vessel, wherein the sample solution includes a known concentration of a sample solution chromophore which absorbs light at a first wavelength, and wherein the sample solution does not include the diluent chromophore; d. mixing the diluent and the sample solution in the vessel to produce a mixture of the sample solution and the diluent; e. measuring the absorbance of the mixture of the sample solution and the diluent at the first wavelength and at the second wavelength; and f. calculating the volume of the sample solution added to the vessel based on the measured absorbances at the first wavelength and the second wavelength. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification