Biopolymer extraction from plant materials

• US 7,923,039 B2
• Filed: 01/05/2006
• Issued: 04/12/2011
• Est. Priority Date: 01/05/2005
• Status: Expired due to Fees

• Current Assignee: Yulex Corporation
• Original Assignee: Yulex Corporation
• Inventors: Martin, Jeffrey A., Cornish, Katrina, McCoy, Raymond G. III, Nocera, Anthony Jr., Williams, Jali
• Primary Examiner(s): Woodward; Ana L
• Application Number:
  US11/327,266
• Publication Number:
  US 20060149015A1
• Time in Patent Office: 1,923 Days
• Field of Search: 210/634, 210/511, 424/725, 424/775, 209/3, 209/12.1, 209/13, 528/1, 528/480, 528/499, 528/502.R, 528/502.A, 528/502.D, 528/930, 528/931, 528/932, 528/934, 528/937
• US Class Current: 424/725
• CPC Class Codes: B01D 11/0284 Multistage extraction
• CPC Class Codes: B01D 11/0288 Applications, solvents
• CPC Class Codes: C08C 1/04 Purifying; Deproteinising
• CPC Class Codes: C08C 1/075 Concentrating
• CPC Class Codes: C08C 2/06 Wining of rubber from solutions
• CPC Class Codes: Y10S 528/93 Guayule rubber
• CPC Class Codes: Y10S 528/931 Physical treatment of natural rubber or natural rubber containing material or chemical treatment of non-rubber portion thereof, e.g. extraction of rubber from milk weed
• CPC Class Codes: Y10S 528/932 Protein removal or conversion
• CPC Class Codes: Y10S 528/934 Latex
• CPC Class Codes: Y10S 528/937 Concentrating, e.g. creaming

Abstract

Disclosed is a method and apparatus for the extraction of high molecular weight biopolymers from plants. Specifically, invention described herein relates to the commercial processing of plant material, including that from desert plants native to the southwestern United States and Mexico, such as the guayule plant (Parthenium argentatum), for the extraction of biopolymers, including natural rubbers. More specifically, the invention relates to laboratory to commercial scale extraction of high molecular weight biopolymers from plant materials including the chemical and mechanical processing of the plants and purification of the extracted biopolymer.

Claims

1. A method for commercial-scale extraction of a biopolymer from a non-Hevea plant material, comprising:

separating small leaves, flower parts and small stems from a non-Hevea plant material, wherein the plant material is guayule, using a, high capacity air density separator, wherein the small leaves, flower parts and small stems are sent to a biomass collection and reuse area;

debarking the plant material, wherein the de-barking system increases the percentage of biopolymer extracted, wherein the woody pulp derived from the de-barking system is sent to the biomass collection and reuse area;

grinding of the plant material;

milling of the plant material in a chemical solution system, the chemical solution comprising a water-based solution to emulsify the plant material to form a slurry, a buffer, an anti-microbial agent, a bleaching agent, a de-foaming agent and an antioxidant, wherein the chemical solution is maintained at a pH between 7.1 and 12.5, whereby the milling results in a milled product comprised of a solid bagasse and a liquid homogenate, wherein the liquid homogenate includes milled plant material, a buffer and water;

filtering out the solid bagasse from the liquid homogenate;

separating the liquid homogenate into a light phase and a heavy phase using a commercial centrifuge;

purifying the light phase, wherein soluble proteins are removed; and

concentrating the light phase, wherein the concentration of the light phase produces a usable biopolymer.

2. The method of claim 1, further comprising chopping the plant material into pieces prior to grinding the plant material.

3. The method of claim 1, wherein the buffer is selected from the group consisting of:

ammonium hydroxide, potassium hydroxide, sodium hydroxide, and sodium bicarbonate.

4. The method of claim 1, wherein the antioxidant is selected from the group consisting of:

sodium sulfite, butylated hydroxytoluene, butylated hydroxyanisole, ascorbate, propyl gallate, alkylated diphenylamine, polybutylated bisphenol A, alkylated para-phenylenediamine, styrenated phenol, and hindered bisphenol.

5. The method of claim 1, further comprising washing the bagasse, whereby additional biopolymer particles are removed from the bagasse.

6. The method of claim 1, wherein filtering the bagasse includes using a screen to separate the liquid homogenate from the bagasse.

7. The method of claim 1, further comprising using the bagasse in a secondary manufacturing process.

8. The method of claim 7, wherein the secondary manufacturing process is selected from a group consisting of:

resin extraction, ligin extraction, cellulose processing and hemi-cellulose processing.

9. The method of claim 7, wherein the secondary manufacturing process produces a product selected from the group consisting of:

resin, ligin, adhesive, pesticide, an anti-fungal agent, ethanol, particle board, insulation, mulch, organic soil amendment, fuel pellets, fireplace logs, ceiling tiles, building material, playground equipment, and flooring.

10. The method of claim 1, further comprising decanting the liquid homogenate, wherein additional solid bagasse is removed.

11. The method of claim 10, wherein decanting includes centrifugation.

12. The method of claim 1, further. comprising removing water from the liquid homogenate.

13. The method of claim 1, further comprising removing the heavy phase to a waste water recovery area.

14. The method of claim 1, wherein purifying the light phase includes mixing it with ammonia and an antioxidant.

15. The method of claim 1, wherein the usable biopolymer is rubber latex.

16. The method of claim 15, wherein the latex conforms to specifications of the American Society for Testing and Materials for a standard selected from the group consisting of:

D1076-02 Standard Specification for Rubber-Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex Type 1; and

D1076-02 Standard Specification for Rubber-Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex Type 3.

17. The method of claim 1, wherein the plant material includes more than one percent of the biopolymer.

18. The method of claim 1, further comprising storing the biopolymer.

19. A method for commercial-scale extraction of a biopolymer from a non-Hevea plant material, comprising:

separating small leaves, flower parts and small stems from a non-Hevea plant material using a, high capacity air density separator, wherein the small leaves, flower parts and small stems are sent to a biomass collection and reuse area;

debarking the plant material, wherein the de-barking system increases the percentage of biopolymer extracted, wherein the woody pulp derived from the de-barking system is sent to the biomass collection and reuse area;

grinding of the plant material;

milling of the plant material in a chemical solution system, the chemical solution comprising a water-based solution to emulsify the plant material to form a slurry, a buffer, an anti-microbial agent, a bleaching agent, a de-foaming agent and an antioxidant, wherein the chemical solution is maintained at a pH between 7.1 and 2.5, whereby the milling results in a milled product comprised of a solid bagasse and a liquid homogenate, wherein the liquid homogenate includes milled plant material, a buffer and water;

filtering out the solid bagasse from the liquid homogenate;

separating the liquid homogenate into a light phase and a heavy phase using a commercial centrifuge;

purifying the light phase, wherein soluble proteins are removed; and

concentrating the light phase, wherein the concentration of the light phase produces a usable biopolymer, wherein the biopolymer is rubber latex, and wherein the rubber latex conforms to specifications of the American Society for Testing and Materials for a standard selected from the group consisting Of;

D1076-02 Standard Specification for Rubber-Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex Type 1; and

D1076-02 Standard Specification for Rubber-Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex Type 3.

20. The method of claim 19, further comprising chopping the plant material into pieces prior to grinding the plant material.

21. The method of claim 19, wherein the buffer is selected from the group consisting of:

ammonium hydroxide, potassium hydroxide, sodium hydroxide, and sodium bicarbonate.

22. The method of claim 19, wherein the antioxidant is selected from the group consisting of sodium sulfite, butylated hydroxytoluene, butylated hydroxyanisole, ascorbate, propyl gallate, alkylated diphenylamine, polybutylated bisphenol A, alkylated para-phenylenediamine, styrenated phenol, and hindered bisphenol.

23. The method of claim 19, further comprising washing the bagasse, whereby additional biopolymer particles are removed from the bagasse.

24. The method of claim 19, wherein filtering the bagasse includes using a screen to separate the liquid homogenate from the bagasse.

25. The method of claim 19, further comprising using the bagasse in a secondary manufacturing process.

26. The method of claim 25, wherein the secondary manufacturing process is selected from a group consisting of:

resin extraction, ligin extraction, cellulose processing and hemi-cellulose processing.

27. The method of claim 25, wherein the secondary manufacturing process produces a product selected from the group consisting of:

resin, ligin, adhesive, pesticide, an anti-fungal agent, ethanol, particle board, insulation, mulch, organic soil amendment, fuel pellets, fireplace logs, ceiling tiles, building material, playground equipment, and flooring.

28. The method of claim 19, further comprising decanting the liquid homogenate, wherein additional solid bagasse is removed.

29. The method of claim 28, wherein decanting includes centrifugation.

30. The method of claim 19, further comprising removing water from the liquid homogenate.

31. The method of claim 19, further comprising removing the heavy phase to a waste water recovery area.

32. The method of claim 19, wherein purifying the light phase includes mixing it with ammonia and an antioxidant.

33. The method of claim 19, wherein the plant material includes more than one percent of the biopolymer.

34. The method of claim 19, further comprising storing the biopolymer.

35. A method for commercial-scale extraction of a biopolymer from a non-Hevea plant material, comprising:

separating small leaves, flower parts and small stems from a non-Hevea plant material using a, high capacity air density separator, wherein the small leaves, flower parts and small stems are sent to a biomass collection and reuse area;

debarking the plant material, wherein the de-barking system increases the percentage of biopolymer extracted, wherein the woody pulp derived from the de-barking system is sent to the biomass collection and reuse area;

chopping the plant material;

grinding of the plant material;

milling of the plant material in a chemical solution system, the chemical solution comprising a water-based solution to emulsify the plant material to form a slurry, a buffer, an anti-microbial agent, a bleaching agent, a de-foaming agent and an antioxidant, wherein the chemical solution is maintained at a pH between 7.1 and 12.5, whereby the milling results in a milled product comprised of a solid bagasse and a liquid homogenate, wherein the liquid homogenate includes milled plant material, a buffer and water;

filtering out the solid bagasse from the liquid homogenate;

separating the liquid homogenate into a light phase and a heavy phase using a commercial centrifuge;

purifying the light phase, wherein soluble proteins are removed; and

concentrating the light phase, wherein the concentration of the light phase produces a usable biopolymer.

36. The method of claim 35, wherein the buffer is selected from the group consisting of:

ammonium hydroxide, potassium hydroxide, sodium hydroxide, and sodium bicarbonate.

37. The method of claim 35, wherein the antioxidant is selected from the group consisting of sodium sulfite, butylated hydroxytoluene, butylated hydroxyanisole, ascorbate, propyl gallate, alkylated diphenylamine, polybutylated bisphenol A, alkylated para-phenylenediamine, styrenated phenol, and hindered bisphenol.

38. The method of claim 35, further comprising washing the bagasse, whereby additional biopolymer particles are removed from the bagasse.

39. The method of claim 35, wherein purifying the light phase includes mixing it with ammonia and an antioxidant.

40. The method of claim 35, wherein the usable biopolymer is rubber latex.

41. A method for commercial-scale extraction of rubber latex from guayule plant material, comprising:

separating small leaves, flower parts and small stems from a non-Hevea plant material using a, high capacity air density separator, wherein the small leaves, flower parts and small stems are sent to a biomass collection and reuse area;

debarking the plant material, wherein the de-barking system increases the percentage of biopolymer extracted, wherein the woody pulp derived from the de-barking system is sent to the biomass collection and reuse area;

grinding the guayule plant material, wherein the plant material was previously chopped into relatively uniform pieces of three to six inches;

milling the guayule with a chemical solution system, the chemical solution comprising a water-based solution to emulsify the plant material to form a slurry, a buffer, an anti-microbial agent, a bleaching agent, a de-foaming agent and an antioxidant, wherein the chemical solution is maintained at a pH between 7.1 and 12.5, whereby the milling results in a milled product comprised of a bagasse and a liquid homogenate, wherein the liquid homogenate includes milled guayule, the buffer and water;

filtering out the bagasse from the milled product;

separating the liquid homogenate into a light phase and a heavy phase using a commercial centrifuge;

purifying the light phase, wherein soluble proteins are removed; and

concentrating the light phase;

wherein the concentration of the light phase produces a usable biopolymer, wherein the biopolymer is rubber latex, and wherein the rubber latex conforms to specifications of the American Society for Testing and Materials for a standard selected from the group consisting of;

D1076-02 Standard Specification for Rubber-Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex Type 1; and

D1076-02 Standard Specification for Rubber-Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex Type 3.

42. The method of claim 41, further comprising decanting the liquid homogenate, wherein additional solid bagasse is removed.