Multi-channel electroporation system

• US 7,923,238 B2
• Filed: 12/06/2006
• Issued: 04/12/2011
• Est. Priority Date: 02/10/2006
• Status: Expired due to Fees

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Ragsdale, Charles W.
• Primary Examiner(s): Bowers; Nathan A
• Application Number:
  US11/567,373
• Publication Number:
  US 20070231873A1
• Time in Patent Office: 1,588 Days
• Field of Search: 435/285.2, 435/288.4, 435/173.1, 435/173.4, 435/173.5, 435/173.8, 435/287.2, 435/288.2, 435/288.3, 435/173.6, 204/403.01
• US Class Current: 435/285.2
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

Systems, methods and apparatus provide flexible and efficient high throughput electroporation systems. An electrical pulse may be transmitted to any number of channels of a multi-channel sample plate. Drivers can provide the selection of which channels to transmit the electrical pulse. To provide efficient transitions between electrical pulses, discharge circuits provide efficient means achieve a desired voltage.

Claims

1. An electroporation system capable of providing an electrical pulse to one or more samples, the electroporation system comprising:

a capacitance;

a charging circuit configured to charge the capacitance;

a first electrical connector adapted to connect with a common pad of a sample plate having a plurality of banks, each having a plurality of wells, wherein when the first electrical connector is connected with the common pad, the first electrical connector is connected with a first electrode of each well of the banks;

a plurality of drivers, each adapted to connect with one of a plurality of separate pads of the sample plate, wherein when a driver is connected with a separate pad, that driver is connected with a second electrode of each well of one bank, wherein a state of that driver determines whether an electrical pulse is transmitted from the capacitance to the one bank connected with that driver; and

a control processor configured to;

prior to transmitting the electrical pulse from the capacitance to a bank, determine a resistance of a sample in the wells of the banks;

based on the determined resistance, select one or more banks for delivering an electrical pulse from the capacitance; and

control the state of the drivers such that the electrical pulse is transmitted to the selected banks,

wherein prior to the selection of the one or more banks to send the electrical pulse, the control processor is configured to determine a number of banks to be selected based on;

an operating range of one or more circuit components of the electroporation system that are used to provide the electrical pulse to the selected banks; and

the determined resistance,

wherein the number of banks is variable, and wherein the possible values for the variable number of banks include one bank and a plurality of banks.

2. The system of claim 1, wherein the control processor is further configured to determine, based on the determined resistance, a maximum number of banks to which the system is capable of delivering the pulse.

3. The system of claim 1, wherein the drivers are operable such that an electrical pulse is transmitted to all of the banks.

4. The system of claim 1, wherein the drivers are connected with the capacitance and the first electrical connector is connected with ground.

5. The system of claim 1, wherein the drivers are connected with ground and the first electrical connector is connected with the capacitance.

6. The system of claim 1, further comprising:

a memory element configured to store parameters for each electrical pulse to be delivered to the banks of the sample plate, wherein the parameters include which banks to deliver each electrical pulse.

7. The system of claim 1, further comprising:

a control processor that independently controls the state of each of the drivers.

8. A method of using an electroporation system to deliver an electrical pulse to a sample plate having a plurality of banks, the method comprising:

prior to transmitting the electrical pulse from a capacitance to a bank, determining a resistance of a sample in the wells of the banks;

a control processor of the electroporation system determining a number of banks to be selected based on an operating range of one or more circuit components of the electroporation system and based on the measured resistance, wherein the number of banks is variable, and wherein the possible values for the variable number of banks include one bank and a plurality of banks;

selecting, with a control processor, one or more banks equal to the determined number of banks for delivering an electrical pulse from a capacitance;

charging a capacitance with a charging circuit;

connecting a first electrical connector with a common pad of a sample plate having a plurality of banks, each having a plurality of wells, wherein when the first electrical connector is connected with the common pad, the first electrical connector is connected with a first electrode of each well of the banks;

connecting one or more of a plurality of drivers with respective separate pads of the selected banks of the sample plate, wherein when a driver is connected with a separate pad, that driver is connected with a second electrode of each well of one bank, wherein the control processor is coupled with the charging circuit and the plurality of drivers; and

subsequent to determining the resistance of the sample in the wells of the banks and selecting the one or more banks, transmitting one or more electrical pulses from the capacitance to one or more selected banks based upon the state of the drivers.

9. The method of claim 8 wherein the control processor is coupled with the charging circuit and the plurality of drivers.

10. The method of claim 8, further comprising:

determining, based on the determined resistance, a maximum number of banks to which a system is capable of delivering the electrical pulse; and

delivering the electrical pulse to no more than the maximum number of banks.

11. The method of claim 8, wherein an electrical pulse is transmitted to all of the banks.

12. The method of claim 8, wherein the drivers are connected with the capacitance and the first electrical connector is connected with ground.

13. The method of claim 8, wherein the drivers are connected with ground and the first electrical connector is connected with the capacitance.

14. The method of claim 8, further comprising:

accessing a memory to obtain parameters for each electrical pulse to be delivered to the banks of the sample plate, wherein the parameters include which banks to deliver each electrical pulse.

15. The method of claim 14, wherein transmitting an electrical pulse from the capacitance to one or more selected banks includes:

transmitting one or more electrical pulses to only a portion of the banks, wherein the portion includes at least two of the banks.

16. The method of claim 15, wherein transmitting an electrical pulse from the capacitance to one or more selected banks further includes:

transmitting different electrical pulses to each of the selected banks.

17. The method of claim 8, further comprising:

controlling the state of each of the drivers with a control processor.

23. The method of claim 12, further comprising:

determining, based on the resistance of the selected banks, a resistance of a variable resistance device that is connected in parallel with the selected banks.

18. A method of using an electroporation system to deliver an electrical pulse to a sample plate having a plurality of banks, the method comprising:

prior to transmitting the electrical pulse from a capacitance to a bank, measuring a resistance of at least one bank;

based on the resistance and on an operating range of one or more circuit components of the electroporation system, a control processor of the electroporation system selecting one or more banks in which to deliver an electrical pulse, wherein the number of banks is variable, and wherein the possible values for the variable number of banks include one bank and a plurality of banks; and

transmitting an electrical pulse to the selected banks.

19. The method of claim 18 wherein measuring the resistance comprises determining whether the resistance is within two or more ranges of resistance values.

20. The method of claim 18 wherein measuring the resistance of at least one bank comprises measuring a resistance of each selected bank.

21. The method of claim 18 wherein measuring the resistance of at least one bank comprises measuring a resistance of each bank.

22. The method of claim 18, further comprising determining a resistance of the selected banks.

24. The method of claim 18, further comprising turning “

on”

a driver coupled with each selected bank.

25. The method of claim 18, further comprising

receiving one or more parameters for the electrical pulse, wherein selecting the one or more banks is also based on the parameters.