Guided mode resonant filter biosensor using a linear grating surface structure

• US 7,923,239 B2
• Filed: 03/14/2008
• Issued: 04/12/2011
• Est. Priority Date: 10/30/2000
• Status: Expired due to Fees

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Li, Peter, Cunningham, Brian T., Pien, Homer, Qiu, Jean
• Primary Examiner(s): Chin; Christopher L
• Application Number:
  US12/048,560
• Publication Number:
  US 20080219892A1
• Time in Patent Office: 1,124 Days
• Field of Search: None
• US Class Current: 435/287.1
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/813 Cancer

Abstract

Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.

Claims

1. A detection system comprising:

(a) a biosensor, wherein the biosensor comprises;

(i) a one-dimensional grating surface structure comprised of a material having a low refractive index;

(2) a high refractive index material layer that is applied on top of the low refractive index one-dimensional grating layer; and

(3) one or more specific binding substances immobilized on a surface of the high refractive index layer opposite of the one-dimensional grating surface structure comprised of a material having a low refractive index;

wherein the one or more specific binding substances do not comprise detection labels;

wherein, when the biosensor is illuminated a resonant grating effect is produced on a reflected radiation spectrum; and

wherein the cross-sectional period of the one-dimensional grating is less than the wavelength of the resonant grating effect;

(b) a light source that directs light to the biosensor; and

(c) a detector that detects light reflected or transmitted from the biosensor.

2. The detection system of claim 1, wherein a polarizing filter is present between the light source and the biosensor.

3. The detection system of claim 1, further comprising a fiber probe comprising one or more illuminating optical fibers that are connected at a first end to the light source, and one or more collecting optical fibers connected at a first end to the detector, wherein the second ends of the illuminating and collecting fibers are arranged in line with a collimating lens that focuses light onto the biosensor.

4. The detection system of claim 3, wherein the illuminating fiber and the collecting fiber are the same fiber.

5. The detection system of claim 1, wherein the light source illuminates the biosensor from its top surface or from its bottom surface.

6. The detection system of claim 1, wherein the one or more specific binding substances are arranged in an array of distinct locations on the high refractive index material.