Photo-activated field effect transistor for bioanalyte detection

• US 7,923,240 B2
• Filed: 03/31/2006
• Issued: 04/12/2011
• Est. Priority Date: 03/31/2006
• Status: Expired due to Fees

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Su, Xing
• Primary Examiner(s): Lam; Ann Y
• Application Number:
  US11/394,156
• Publication Number:
  US 20070231790A1
• Time in Patent Office: 1,838 Days
• Field of Search: 422/50, 422 821- 823, 435/287.2
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

The application relates to a device and method for performing analyte detection, especially biomolecule detection. The device and method combine photo-induced charge separation in label materials and field effect transistors as sensors, resulting in more sensitive, specific and/or selective detections of biomolecules in multiplex assays, such as immunoassays and DNA microarray assays. The embodiments of the invention also encompass a device and method that comprise an array of electrical sensors, such as field effect transistors, and binding complexes for simultaneous multiplex detection of analytes.

Claims

1. A device comprising an electrical sensor and a complex associated with a surface of the electrical sensor, wherein the complex comprises a detection probe and a label bound to the detection probe, the label being capable of absorbing radiation and separating an electrical charge upon being exposed to radiation, the label comprising a first material of a band gap Eg and a second material of band gap Eq that is smaller than Eg such that the label is configured to decouple absorbing radiation from the separating the electrical charge, and wherein the electrical sensor is capable of detecting the electrical charge.

2. The device of claim 1, wherein the electrical sensor comprises an exposed electrode surface to detect electrical perturbation, impedance, current or voltage.

3. The device of claim 1, wherein the electrical sensor comprises an electromagnetic sensor, a transistor, an electrical resistance sensor, an electrical power sensor, a magnetism sensor, an electrical current sensor, or an electrical voltage sensor.

4. The device of claim 3, wherein the FET is a metal-oxide-semiconductor FET (MOSFET), a junction FET (JFET), a metal-semiconductor FET (MESFET), or a high-electron-mobility (HEMFET).

5. The device of claim 1, wherein the electrical sensor comprises an ohmmeter, a multimeter, a galvanometer, an ammeter, a leaf electroscope, a voltmeter, a watt-hour meter, a magnetic compass, a fluxgate compass, or a magnetometer.

6. The device of claim 1, wherein the electrical sensor comprises a field effect transistor (FET).

7. The device of claim 6, wherein the FET comprises a nanowire, a nanocrystal, a nanotube, a nanopillar, a nanogap, or a patterned nanostructure.

8. The device of claim 7, wherein the FET comprises a single-walled carbon nanotube.

9. The device of claim 6, wherein the surface of the FET is functionalized.

10. The device of claim 6, wherein the surface is on the gate area of the FET.

11. The device of claim 1, wherein the complex comprises a first binding partner immobilized on the surface of the electrical sensor and an analyte bound to the first binding partner, and wherein the label is bound to the analyte.

12. The device of claim 11, wherein the analyte comprises a biomolecule.

13. The device of claim 12, wherein the analyte comprises an antigen, an antibody, a protein, a peptide, a virus, a bacterium, a carbohydrate, a lipid, a polynucleotide, a nucleic acid or a macromolecule.

14. The device of claim 11, wherein the first binding partner comprises a biomolecule.

15. The device of claim 14, wherein the first binding partner comprises an antibody, an antigen, a receptor, a ligand, a protein, a peptide, a virus, a bacterium, a carbohydrate, a lipid, a polynucleotide, a nucleic acid or a macromolecule.

16. The device of claim 11, wherein the analyte comprises an antigen and the first binding partner comprises an antibody to the antigen.

17. The device of claim 11, wherein the analyte comprises a peptide and the first binding partner comprises a receptor or ligand to the peptide.

18. The device of claim 11, wherein the analyte comprises a first polynucleotide and the first binding partner comprises a complementary polynucleotide of the first polynucleotide.

19. The device of claim 1, wherein the complex comprises a first binding partner immobilized on the surface of the electrical sensor, an analyte bound to the first binding partner, and a second binding partner bound to the analyte, and wherein the second binding partner comprises the detection probe.

20. The device of claim 19, wherein the analyte comprises a biomolecule.

21. The device of claim 20, wherein the analyte comprises an antigen, an antibody, a protein, a peptide, a virus, a bacterium, a carbohydrate, a lipid, a polynucleotide, a nucleic acid or a macromolecule.

22. The device of claim 19, wherein the first binding partner or the second binding partner comprises a biomolecule.

23. The device of claim 22, wherein the first binding partner and the second binding partner independently comprises an antibody, an antigen, a receptor, a ligand, a protein, a peptide, a virus, a bacterium, a carbohydrate, a lipid, a polynucleotide, a nucleic acid or a macromolecule.

24. The device of claim 19, wherein the analyte comprises an antigen and the first and second binding partners independently comprise an antibody to the antigen.

25. The device of claim 19, wherein the analyte comprises a peptide and the first and second partners independently comprise a receptor or ligand to the peptide.

26. The device of claim 19, wherein the analyte comprises a first polynucleotide and the first and second binding partners independently comprise a complementary polynucleotide of the first polynucleotide.

27. The device of claim 1, wherein the label comprises a quantum dot, a quantum well, a semiconductor nanoparticle, a composite-organic-inorganic nano-cluster, or a combination thereof.

28. The device of claim 1, wherein the electrical charge comprises an electrical perturbation, impedance, current, voltage, or a photo-induced charge separation.

29. The device of claim 1, wherein the radiation is electromagnetic radiation.

30. The device of claim 29, wherein the radiation is electromagnetic radiation in the form of terahertz radiation, infrared, visible light, ultraviolet light, or X-rays.

31. The device of claim 1, wherein the electrical sensor is associated with a substrate.

32. The device of claim 31, wherein the substrate comprises a polymer silicon or glass.

33. The device of claim 31, wherein the substrate comprises a microarray, a macroarray, a multi-well plate, a microfluidic device, an integrated circuit, a micro-electro-mechanical system (MEMS) or a combination thereof.

34. The device of claim 1, further comprising a microprocessor capable of processing signals or data detected by the electrical sensor.

35. The device of claim 1, wherein the first and second materials comprise semiconductors.

36. The device of claim 1, wherein the label further comprises a conductive material.

37. The device of claim 36, wherein the conductor is a conductive polymer.

38. A device comprising an array of electrical sensors and a complex associated with a surface of each of at least a portion of the electrical sensors, wherein each said complex comprises a detection probe and a label bound to the detection probe, said label being capable of absorbing radiation and separating an electrical charge upon being exposed to radiation, the label comprising a first material of a band gap Eg and a second material of band gap Eq that is smaller than Eg such that the label is configured to decouple absorbing radiation from the separating the electrical charge, and wherein the associated electrical sensor is capable of detecting the electrical charge.

39. The device of claim 38, wherein at least a portion of the electrical sensors are field effect transistors (FETs).

40. The device of claim 38, wherein at least a portion of the electrical sensors are individually addressable.

41. The device of claim 38, wherein each of at least a portion of the electrical sensors is associated with one or more complexes.

42. The device of claim 38, wherein a single complex is associated with two or more of the electrical sensors.

43. The device of claim 38, wherein the complexes associated with a single electrical sensor comprise the same analyte.

44. The device of claim 38, wherein at least two of the complexes associated with a signal electrical sensor comprises different analytes.

45. The device of claim 38, wherein the complexes associated with at least two of the electrical sensors comprise the same analyte.

46. The device of claim 38, wherein the complexes associated with at least two of the electrical sensors comprises different analytes.

47. The device of claim 38, wherein each of at least a portion of the electrical sensors is associated with a single complex.